Sequence retrieval

Genome: Alcanivorax dieselolei B5
Start position: 450520
End position: 453576
Length: 3057

DNA sequence
>NC_018691, from 450519 to 453575 (3057 bp); Alcanivorax dieselolei B5 TTAGCGGGCCGGACGAGGAACCCTGAACCAGGCGGCGTACAGCGCGGGCAGAAACAGGAGCGTCAGGGCA GTGGCGACGATCAGGCCGCCCATGATCGCCACGGCCATCGGGCCGAAGAAGTCGCTGCGGGACAGCGGGA TCATCGCCAGCACCGCGGTGAGCGCGGTGAGGGCGATAGGCCGGAAGCGCCGTACCGTGGATTCAATGAT GGCTTCGTATTGGCCGCGTCCCTGGGCCAGATCCTGGCCGATCTGATCCACCAGAATCACCGAGTTGCGC ATGATCATGCCGGCCAGCGCGATGGTGCCCAGCATGGCGACGAAGCCGAACGGTTGACCGAAGAACAGCA GGAACAGGGTGACGCCGATCAGCCCCAGGGGCGCGGTCAGGAACACCATCACCGTCAGCGAGAAGCTGCG CAGTTGCAGCATCAGCAGCGTCAACACCACCAGCACGAACAGCGGCATGCCGGCGTTCACCGATTTCTGG CCGCGGGCGGATTCCTCCACGGTGCCACCTACTTCCAGCAGGTAGCCCGGTGGCAGGGCATCACGGATAT CCTGCAAGGTGGGCTGGATCTTGTTAACCAGAGTGGCGGGCTGTTCGTCGCCGTAAATGTCGGCGCGGAC AGTCACCGTCGGCAAACGATTGCGGTGCCAGATGACGCCTTCCTCGAAGCCGTATTCCAGGGTGGCGATC TGGGACAGCGGCACACTGCGGCCGTCCTCGTTCTGCACCGCCAGACTCGGTAACAGCCCCAGTGCTTTGC GCTCGCGGGCATCGCCACGGACTTCCACGTCGATCAACTCGTTGTCTTCCCGGTACTGGCTGACCGCCAC CCCGGACAGCGAGCTTTGCAGAGCCTGGGACAGATCGGTGGTGTTGACGCCGAGCGCCCGGGCACGGTCC TGGTCGATGTTCAGGCGCACCACCTTGCTGGGTTCTTCCCAGTCCAGATGCACGTTGCTGACCTGCGGTT GCTCGCGTATTCGTTCCGCCACCTGGCGGGCCAGATCACGAACTTCGCCGATGTGCTCCCCGGATACCCG GAACTGGATCGGGTAGCCCACCGGCGGGCCGTTCTCCAGACGCGACACCCGCGAACGCACGGTGGGGAAG TCCGCCGCCAGCGTGTCGATCAGCCAGGAGCGAACTTCTTCCCGCTCCTCAATATTGCGGGTCAGCACAA CGAATTGGGCGAAGCTCGCCGCCGGCAGTTGCTGGTCCAGAGGCAGATAGAAGCGGGGAGATCCGGTGCC GACATACGCCACGTAGTTCTCCACCTGGTCGTTTTCCGCCAGCAGGGCTTCGAGCCGCTCCGCCTGAGCC TGACTGGCTTCCAGTGAACTGCCTTCGCTCAGCTTCAGATCCACCATCAGTTCCAGTCGCGCGGAAGGCG GGAAGAACTGTTGCGGCACGAAGCGAAATAGCGCCACCGCGCCAATGAAGATCGCCACCGTCATCACGAT CACGGTTTTGCGGTGGTAAACACACCAGCTCACCAGCGCGCGGAAACGGCGGTAAAACGGCTTCTGGTAG GGGTCGTGAACTTCACCGGCGTCATTGCGGCGCGCCAGATTGGGCAGCAGCTTGTCGCCCAGGTAAGGCA CGAACACCACCGCGGTAATCCAGGACACCAACAATGACAATGTCACCACCTGGAAAATGGATCGGGTGTA CTCGCCGGTGCCGGACTGGGCCGTGGCGATCGGCAGAAAGCCAGCCGCGGTAATCAGGGTGCCGGTGAGC ATGGGGAAGGCGGTACTGGTCCAGGCGAAACTGGCCGCCTTGATCCGGCTCAGACCCTGTTCCATCTTGA TCGCCATCATCTCCACCGCGATGATGGCGTCGTCCACCATCAGCCCCAGCGCCAGCACCAGCGCCCCGAG CGAAATCTTGTGCAGGCCGATGTCGAAGTAATTCATCAGCGCGAAGGTCATCGCCAGGACCAGGGGAATG GATAACGCCACCACCAGACCGGTGCGCAGTCCCAGCGAGAAAAAACTCACCAGCAGCACGATCACCAGGG CCTCCACCAGCACCCGCACAAAGTCGCCGACGCTGGCGCGTACCGCCGCCGGCTGGTCGGAGACCTTATG CAGTGACAGGCCAGCCGGCAGAGTGTCCTGCAGGCGCGCGAATTCCTTTTCCAGGTTTTCACCGAGGGCG AGAATGTCGCCGCCTGCCTTCATGGAGACCGCCAGGCCAATGGCGTCCTCACCCATGAAACGCATGCGCG GTGCCGGCGGGTCGTCGAAGCCACGGTAGACCTTGGCGATGTCCTCCAGGCGCAGGGTGCGGCCCTCGAA TCGGATGGGGAAGCGGCGGATCTCCTCCACGCTTTGGAAATTACCGGAGGCGCGCAGGCGCACGCGGTCG GAGGGTGTCTCAAAATAACCGGCGTCGGAAACCGCGTTCTGTTGTTGCAGGGCCTGTTGCACCGCGCCGA GCGGAATGCCGAGGGCGGCCAGCTTGGTATTGGACAGCTCTACCCAGATTTTCTCGTCCTGCAGGCCAAG CAGCTCCACTTTGCCCACGTCCTTGACCCGTTGCAGGGCCAGTTGCAGGCGGTCGGCGTAGTCTTTCTTC AGCGCGTAATCGAAGCCGTCACCGGTGAGCGCGTAGATATTGCCATAGGTAGTGCCGAACTCGTCATTGA AGAACGGGCCCTGGATACCCTGGGGCAGGGTGTGGCGGATATCGCCCACCTTCTTGCGCACCTGATACCA GAGATCGGGAATGTCGTCGGAGTGCATGTCGTCCCGCGCCATGAACATGACCTGGGACTGACCGGGGCGG GAAAAGGAGGTGATGCGGTCATAGTCCCCGGTTTCCATCAGCTTCTTTTCGATACGCTCGGTGACCTGAC GGGAAACGTCCTCGGCGCTGGCGCCGGGCCACAGCGTGTTCACCACCATGACCTTGAAGGTGAACGGCGG GTCCTCGCTTTGTCCAAGCTGGCTGTAGGAAAGCGCGCCGAGGGCGCCCAGAAGGATCATCGCGTAGAGC ACCAGGGCGCGGTTGTGCAGCGCCCATTCGGACAAATTGAAACGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 450520-453576	Sequence	B5T_00439 - RND transporter, HAE1/HME family, permease protein (Protein is coded in complementary strand; from 453576 to 450520)