Sequence retrieval

Genome: Alcanivorax dieselolei B5
Start position: 3595411
End position: 3596220
Length: 810

DNA sequence
>NC_018691, from 3595410 to 3596219 (810 bp); Alcanivorax dieselolei B5 TCATTTGGCATCTCCAGGTTCAAATTCGAGCACAAGCGTTCGGAACTCTTCAAAACCGTCGCCTTTCGGC ACCGGCAGGGGGCTGGCCAGTTGTACGCCCTTGACCACGGAATCATCGAAGTCCGGATAACCGCTGGACT TCACCACATCCACATTGACCACGTCACCGCCGGGAACCATCCGGATACGTACCCGGGTACGGATGTCGTT CCGGTTACGGTAGTTGCCGGGAATGCGCCAGCGTTGGGCCACCGCCGAGGAGATGGCGTCAATATAGCTG GCGGTTTCCTGTTCCACCTTGCTGTCCGGGTCACCGCTGCCGGGATTCTCCTGGTCCTGCTTCTTACCCT GACGCGCCATCTCCAGCCGTTCCTGAGCCAGCAGTTCCTCCATGTCCGGCTGCTCGAACTTGAGCGGTGG CTCCGGTTTCGGTTTCGGTTTCGGTTCCGGTTTTTTGGGTTCCGGTTTGGGTTCGGGCTTCGGTTTCAGC TCTGGCTTAGGCTCGGGTTTCGGTTTGGGTTCCGGCTTCGGCTCAGGCTTGGGCTTCGGTTCCGGCTTCG GTTCCGGCTTAGGCTCCGGTTTAGGTTTCGGCTTGGGCTCTGGTTTGGGCTGCGGCGTCTCTTGCTTGGG CTCGGGACGGGTGGGCGCCGCCGCCCGCTCGGTCTGGTCGGTAACCACCGCCATCACATGAGGAGGAATC TTCGGGGGACGGCGCAGTTCCGGCTCGGAGGTCCAGGTAAACAGCACCATTCCCAACACCAACAGGTGCA GGAACAGGGACAGCCCCGCGCCTACCCCACGGTCACTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 3594137-3595414	Sequence	B5T_03226 tolB Protein tolB (Protein is coded in complementary strand; from 3595414 to 3594137)
ORF.. 3595411-3596220	Sequence	B5T_03227 - Protein TolA (Protein is coded in complementary strand; from 3596220 to 3595411)
ORF... 3596217-3596600	Sequence	B5T_03228 tolR Biopolymer transport protein TolR (Protein is coded in complementary strand; from 3596600 to 3596217)