Sequence retrieval

Genome: Alcanivorax dieselolei B5
Start position: 1685966
End position: 1686436
Length: 471

DNA sequence
>NC_018691, from 1685965 to 1686435 (471 bp); Alcanivorax dieselolei B5 TCAACTATCAACTGTTTTATACGGTGTTTTGCTCAGAGCGCTGTTGTAGTAGCGCGGGTCGCCGGTGACC TCGTTGCCGATCCACGGGGGCAGGGGGAAGGTCTGGTCTTCGTGGTCCAGTTCCAGTTCGGCGACGATCA GGCCCTGGTTTTCGCCGTGGAAGACATCCACTTCCCAGGTGTGGCCCTGGTGGGGAACCAGGTAGCGGGT TTTGTCGATGACGCCTTCGCCGCACAGTTCGTCGAGCATGGCGCGGGCGTCCCGCGGCGGGATCGGATAT TCGAATTCGCTGCGGCTGGCGCCGTGGGTTTCCCCCTTGATGGTGAGCCAGGCGCTGTCGCCCTTGAGCC GCACCCGCACCGTGGCCCGCTTGTCGTGGCTGAGATAGCCCTGGGTCAGACGCTCGCCGTCGAGGGTATC GAGAAAGGCACTGTCGCGGACCAGGAATTTGCGTTCGATTTCGATGCCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1685966-1686436	Sequence	B5T_01571 - Adenylate cyclase (Protein is coded in complementary strand; from 1686436 to 1685966)