Sequence retrieval

Genome: Alcanivorax dieselolei B5
Start position: 3594137
End position: 3595414
Length: 1278

DNA sequence
>NC_018691, from 3594136 to 3595413 (1278 bp); Alcanivorax dieselolei B5 CTACAGAAACGGCGACCAGGCCGGTTCCCTGACTTCCCCTTCCTTCGACGGCAGCTCTACCTTGACTCTG CCATCAATGGATACGGCCTCCAGGACCCCCGTGCCGCGACGTTGCGTACCGTAAATTACCATACTTCCAT TAGGCGCAACACTGGGCGACTCATCCATATTGGTCTGAGTCACAATATCGAACGTACCCCGCTGCAGGTC CTGCACGCCGACGTTGAAACTGCCGTTGCGACGGTGCACATAAACCAGATACCGGCCGTCCGGGGTGACA TCCGGACGGGCGTTGTAATTGCCCTCGAAGGAAACCCGCCGGGCCGATTTGTCGTTGATGTTGAGCATGT AGATCTGCGGGCCACCGGAACGGCTGGACGTGAACACCACGTGCTCGCCGTCCGGCATCCAGCGCGCCTC GGTATCGATACCGTAGTTGTCCGTCAGCCGGGTCAACCCGCCGCCGTTCACATTCACCATATACAGCTCC GGATTACCATCCTTGGACAGGGTGATCACCAGACGCTGACCGTCCGGTGACCAGGACGGAGCGCCGTTGA TACCCCGCTCCCGGCTCACCACCGAGCGCTGTCCGGACGCCAGATTGTGCACGTAGATGTTCGGCTTGCC GTTGTTCTCGAAGGAGACATAGGCGATCTTGCTGCCATCCGGTGACCAGGACGGGCTCATGATCGGCTCG GCACTGGACAGAATGGTCTTCACCCGATGGCCGTCGGCATCGGCGTACTGCAGTTGATAAGGCAGCTTGG CGCCACGGTTATGGGTGACATAAAGGATCTTGGTGGAAAAGGCACCGGGAATGCCGGTGAGCTCTTCATA GATGCGATCGGAAATGGCGTGGGCCACATCCCGCAATTGAGCTTTGGTGGGACTGTAGCTTTCGCTGAAC AGTTCACGCTGGCGACCCACGTCATACAAGCCATAGGTCACTTCATAGCGGCCATCGTCCCGCGGCTTGG TATGGCCGATCACCAGATACTCCTGGCCGAGCACACGGAAATCCCGGTAAATGATCGCGTCCTTGGTGTG CGGCCGGCTGAGCATGTTCTTGGCCGGTAGCGGTTTGAACTGACCACTGCGGTGCAGGTCCGCCTCGACA ATGGAGGACACCTGCTCCGGCGCCTGGTTGTCGCCGTCAAACGGCACGATGGCGATCGGCACCGCGTCCA CCCGTCCTTCGGTGACCTTGATCAACAGCTCGGCCCGCGCGGCCACCGTCAGTGTCGCCAGGACCAGGGC CAATATCAGTCGCTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 3594137-3595414	Sequence	B5T_03226 tolB Protein tolB (Protein is coded in complementary strand; from 3595414 to 3594137)
ORF... 3595411-3596220	Sequence	B5T_03227 - Protein TolA (Protein is coded in complementary strand; from 3596220 to 3595411)