Sequence retrieval

Genome: Brachyspira pilosicoli B2904
Start position: 371679
End position: 372962
Length: 1284

DNA sequence
>NC_018607, from 371678 to 372961 (1284 bp); Brachyspira pilosicoli B2904 TTAAAAAATATCTTTTATTATGATAGTTTCATCTCTTCCAGCTCCAACAGACACTATTGATATTTCTGTG TCTATTACTTCGCTTAGTCTTTTTAAGTATTTTTTAGCATTTTCTGGAAGCTTATCATATTCTCTAATGT TTGTAGTGCTTGTTTTCCAGCCGTCAAACTCTTCATATACAGGTTCAACTTTTGAAAGTATATCCAAATC AGCTGGATAGTCTTCTAATATTTCTCCATTATATTTGTATGCAACGCATATTTTTAGCTTTTCTAATTCA TCTAATATATCGAGTCTAGTAATTGCTACAGAATCAAGCCCATTTATGTATGAAGCATATTTTATAACGC AAGCATCAAGCCAACCGCATCTTCTTGCTCTTCCTGTAACAGTGCCGTATTCCCCGCCTTTGTCTCTTAT AAACTGTCCTACATCATCGAAAAGCTCTGAAGGGAAAGGTCCTTCTCCAACTCTTGTAGAATAAGCCTTA ACAACTCCTATAACATTATCTATCTTTCTAGGTCCAACTCCAGAACCAGTACAAGCACCGCCCGCAGCAG GGTATGATGATGTTACAAATGGGTAAGTTCCATGGTCTAAGTCAAGCATAGTAGCCTGAGCACCTTCAAA TAATATGTTTTTCTTTTCTTTTATAGCTTTGTTTAATATTGTAGTAGTATCAGCAACATAAGGTCTTAAT CTCTCAGCATATTCCAAATACTCTTTTAATAACTCTTCGTAATTAATTCCATCATGATTATAAAGCTTTT TAAGAAGTTTGTTTTTAAGTTCAACATTAAACTTTAATTTTTTAGCAAATACTTCTTTATTCATTAAATC AACTATTCTTATACCCAAACGGCTTGCTTTATCCATATAACAAGGACCTATTCCGTTTTTAGTAGTGCCG AGTTTATTTTCACCCAAATCCTCTTCCTGAAGTTCATCTAATATTTTATGATAAGGCATAAGAACATGAG CTCTGTTAGATATTTTTAAATTAGACATATTAACTTTTTTTTCTATAAGACCGTCAATTTCACTTAAAAA CACCTTAGGCTCTATTACAACACCATTACCTATAATGCATACTTTATCTTTATGAAGTATACCAGAAGGA AGAAGCCTTAATTTATATTTTACATTATCAACAACAACGGTGTGTCCTGCATTACTTCCGCCCTGAGAAC GAACTACATATTGAGCCTTATTAGCAAGATAATCAACAATTTTTCCCTTGCCTTCATCACCCCACTGTGT ACCTACAATAATAACTGAAGCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 371679-372962	Sequence	B2904_orf391 - adenylosuccinate synthase (Protein is coded in complementary strand; from 372962 to 371679)