Sequence retrieval

Genome: Brachyspira pilosicoli B2904
Start position: 315641
End position: 316450
Length: 810

DNA sequence
>NC_018607, from 315640 to 316449 (810 bp); Brachyspira pilosicoli B2904 TTATATAGAAAGCTTAGAGGTAAATACATATAGGTTGTATATACTTATAAATATTATTAATATTAATATT ATAATAAATATTTTATCAAAGGAACTTGAGTTTAATTTTTTGCTAATTTTTCTTCCGATAATTCCTCCTC CTATTCCTCCTATAATCATTACTATTAATATGTATATGTTGATATTTTGTATTTCATTTTTTATAAGGGT TGTAAGTACGCTAGATATTTGAGAAAATAATATAATATATAATGAGTTTTCTGCGGCTGTTTTATTATCC ATAGCAAAAAATATATATAGGAAGGCTATATTAAAAGGACCGCCTCCTATACCCAAATATGAAGAGAGTA GCCCCAAAACCATACCAATTATAGCAGATAAAAATATATTATTAATATGCATTGGTTTTATTTTATTTTT TGACATATTTTTTATTGTAAACATTACAGTAAAGATTAAAAGTATTATTAATATAGAAGATTGTATAATA CCGGCAATATTTTGATTATTAAAGAAGTGTATAGTATAAGAAAATAGTATTTTTCCTATTAGTCCGCCCA ATACGGCACCTAATGCAAATGGAGTAGCTATTTTTGTATCGACTAAGCTAGTTTTTGATAATTTAGAGTT TATAAATGTATATAGGCTCATAGATAGTACAGTGCATGATGAAAGAAAACTTATTTTTGTAACTTCCATT ATATTAAGGGAATCTAATATTGGTTTTATTATAACTCCGCCGCCTATACCAAATACAGCTCCTATAGAAG AAGCGAATAAACTTACTATAAAAAATATTAGCACAAGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 314480-315673	Sequence	B2904_orf325 - ROK family protein
ORF.. 315641-316450	Sequence	B2904_orf326 - transmembrane protein (Protein is coded in complementary strand; from 316450 to 315641)