Sequence retrieval

Genome: Brachyspira pilosicoli B2904
Start position: 169853
End position: 170587
Length: 735

DNA sequence
>NC_018607, from 169852 to 170586 (735 bp); Brachyspira pilosicoli B2904 TCATTCATTGTTATCTTCCTTTATAAAATCTGTATCTATGCTTGTTATCTTAAAAAACATATCTTTATCA AATTCTTCTAAAGACATCACATATTTAATTGCCGCAATCGGCATATCTTTCCAAGCTTCTTCTTTTTGTA TTTCTTCAGCATTTTGAATCGGTGTTAACTTCCACTCACTGCCGAATTTTAAATAAAGACTTTTAAGATT GTTAAAAGTTGGTCTCCAACCTTTTAACAAATTATAGAATTCTGTTTTAACTTCTTTATATCTTATTTTT GATACTTCTGTACCGAATATAGAATAAGTTTCTTTTTTATCTGTCAAAAACAAAGCATTAGAAACACCTT TACAATTTAATACACCAAAAGAACAATTTACACCATCAGAACCATTTACACCAAAAGAACGATTTACACC ATTAGAATTATTTACAATCTCTTCAAATGTAACTTCTTCTAATATTTTTAATTTATTAGTACAAGACTTA TTATCTTTTTTAATAACATCTCCTAATATTTCTACTTTATAAACTTTTACCCATTGTACTAACGGATAAT AATCAAATACATCTTTTAAGTTTTCGCAAAAATGTATTCCATTTTTACAAATCTTTAATTCCCCATTTAT TTCACAAATTTCACCTTCTTTATATCTGTATTTTTTACCATTAGTACAAGGCTGACAATATAATCCATTT TTGTCTTGATAAAATGCTTTATATCCTGTTATCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 169717-169860	Sequence	B2904_orf181 - hypothetical protein (Protein is coded in complementary strand; from 169860 to 169717)
ORF.. 169853-170587	Sequence	B2904_orf182 - hypothetical protein (Protein is coded in complementary strand; from 170587 to 169853)
ORF... 170584-170760	Sequence	B2904_orf183 - hypothetical protein (Protein is coded in complementary strand; from 170760 to 170584)