Sequence retrieval

Genome: Amycolatopsis mediterranei S699
Start position: 4297562
End position: 4300468
Length: 2907

DNA sequence
>NC_018266, from 4297561 to 4300467 (2907 bp); Amycolatopsis mediterranei S699 TTGCTGGAAGCCACGGTCCTCGGTCCCGTCGGCGCGCGGGTCCACGGAACACCGATCCCGATCAAGGGAC GGCTGGGGCGTACGGTCCTCGCCGTGCTGGCGCTGGCCTGCCGGCAGCCGGTGACGGTGGAACGGCTGAT CGACGCCCTCTGGGGCGAACTGCCGCCGGCCACCGCGCGGACCCAGGTGCACATCCAGGTGTCCAAGCTG CGTGCCTCGCTCGACCGGACGGGCGCCGCCGGTGCCGTGGTCACCGTGGCGAGCGGGTACCTGCTGGACT GCCTGGTCGACGTCGACGAGGTGCGGCGGCTGCTGAGCGCGGCGCGGGCGGCCGGGGATCCCGAGGACAC CGCCGGCCTGCTGCGGTCAGCGCTCGACCGGTGGGCCGGGACGCCGCTGGGCGGGGTGACCCCGTACCTG GCCGGTGCGGAGCTGCTCCGGCTGGACGAGCTGCGGATGTGCCTGGTCGAGGAGTGGATCGACGCGGAGC TCGCGTGCGGGCGGGCCGCGCGGCTGCTGCCCGAGCTGAGCGCGCTGGTGCAGGCGCACCCCCTGCGCGA GGGCCTGCACCACCGGCGGATGCTGGCGCTGCACCGCGCCGGGCGGCGGGCCGACGCGCTGGCGGCGTTC CACGAGGCCCGGCGCGTGCTGCGGGACGAGCTGGGAGTGGATCCGTCCCGGGAGCTGCTCGCACTGGAGG CGGAGATCCTCGCCGTCGGGACGCCTCCGGCGGCCGCCGTGGTGCCCGCCCAGCTGCCGCCGCGGACCTC CGGGTTCGCCGGGCGGGCGGCGGAGTCGGCCGCGATCAGGGCCGCGGTGACGCGCCGGACCGACGCGTCG CCGCTGGTGCTGGTCACCGGCATGCCCGGAATCGGCAAGACGGCGCTCGCCGTGCACGCGGGCGCGGCGT CGGTGCACACCTACCCGGACGGGCAGCTCTACGCCGACCTGCGGGGCGCCGATGCCCGGCCGGCCGCCCC GCACGACATCCTCGCCGGCTTCCTGCGCGCACTGGGGGTGCCGGCGCGGTCGATGCCGGCCACCGTCGAG GAGCGCACGGCCGAGTTCCGCAGTCGCACCGCGGGCAAACGGGTGCTCGTGGTGCTCGACAACGCCGCCA CCGCCGGGCAGATCGAGCCGCTCCTGGTCGCCGAACCGGGCTGCGCCACGATCGTGACAAGCAGGTCCGT GCTGCCCGACGTCGAGTCGGCGGTCCGGGTGCCGCTCGGGCCGCTGCCGGACGAGGTCGCCGGGACGGTA CTGGCGAACGTCGCGGGCGCGGCTCGGCTGGGCGACGGCGACGGCGACGGCACTGCCGATGGCAACGGCA GTGGCAACGGCAATGGCACGGTCGCCACGGTGATCGAGGCGTGCGGTGGCGTACCGCTCGCGTTGCGGAT CGCCGGGGCGCAGCTCGCCGCGTACCCCAGGCTCAGCGTGGCGGAGCTCGCCGCGCGGCTGTCCGACGAG TCGAGGCGGATGCGGGAGCTGGTGGCCGGGCAGCGCAGCGTGCGGCACAGTCTCGACACCAGCTTCCGGT TGCTGGACCGCAACGCGCGGGCCGCGATGCTGGCCCTGGCGGCGATCGGAGCGCCCACGTTCACCGGCTG GCCGCTCGCGCCCGTGCTGGACGTGCCGCTCGCCCGGACCGACGAGGTGCTGGCCCACCTGTGTGCGATG AACCTGCTCGACCAGGCCGGCCCGGGGCGCGACGGGGTGCTCCGCTACCGGATGCACGACCTCGTGCGGG CCTACTGCCGGACGGTCGGCCCGTCGCTCGACCCGGGCCCCCTGAACCGGCTGGCCGGCTGGGCGGTCGC GCTGACCAGGCACGCCCACGAGCGCATGTTGGGCACCTCGGCCCGCTACACCGTGCTCACCGAGGCCGCC GCGGCACTCGGCCAGGACGTGGTGCGGCGCGTGGTGACGGAGCCGCTGTCGTGGGTGGGCGCGGACGTCG ACGTCCTGCTGGCCGTGTTCGCCCTGGCCGTGCGCGGCGGCCTGCTCCGTCCCGCGGCGGCGCTGCTGCT GGTGCTGCGGGCGCCGCTCGTGCGGCTCGACCTGTACGACCACGGCGCCCGGGCGGCCGCGCGGCTGACG GCCGTCGCCGGCCCGGACCCGGTGGTGCGGGTGACCGCGGCGCTGGTCACGGCGACGGCGCGGATGCACC GCAGCCGCTACGCCGACGTCGTCGCCATCCTGAACGAGGTGCCGCTGCGAGACGTCGACTTCGCGCTGCG GGCGGAGGTGCTGACCAAGCTGGGCGACGCGCACGAGCGGTGCGGCGACTGGCACCGCGCGATCACCGCC GTGCGCGATGCGGTCGTGTGCTGCCGGAAATCCGGCAACCTGGCGGGGATGTCGGGCTGCCACGGCACGC TGGCCGCCCTGTACCGGGACCACCTCGGCGATCTCGCCGCGGCGAGGGTGCACGGCGCGAAGGCCTTGCG GCTGGCCGACGCCGCGGGCGACTGGAAGACCTGCGCCCAGGTGCGGCTCGTGGCCGTGCGCACCGCACTG GCCTGCGGCGACGCGGCGGCCGCGGTGAATCTCGCCGGACAGGCTCGCGGCCTGACCGAGCAGCACGGCG ATCCGATCGGCCAGACCTGGTGCTTCACCGCCGAAGCCGACGCCCACCGCGCGAACGGCGACCTGCCCAC CGCCCGGAGAGCCGCGGACCGCGCGCTGGGCCTGGCCCGCCGCACCCGCCGTCCGGACGCCGAGTCCGCC GCGCTGCTCGCGCTGGCCTCGATCGAGCTGGCTGCCGGGGACCGGGCCGCCGCCCGCCGCCTCGCCCACG CGGCCCTGCTCCTGCAGGACCGGTTCGACAGCCCGGTCGAACGCGTCGGCGTGGAGCGGGTGCTGGCGGA GATCGATGGTCAGCTGCCCTCGGGCGCGGCGCGGTGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 4297562-4300468	Sequence	AMES_3893 - SARP family transcriptional regulator
ORF... 4300441-4300848	Sequence	AMES_3892 lepB signal peptidase I (Protein is coded in complementary strand; from 4300848 to 4300441)