Sequence retrieval

Genome: Enterococcus faecium DO
Start position: 1786768
End position: 1788615
Length: 1848

DNA sequence
>NC_017960, from 1786767 to 1788614 (1848 bp); Enterococcus faecium DO TTAATTCATCTTGAGATAACTCTTCACTAAGTTTTTGATACCTCTTTGATACCTCTTTGAAACCTCATTA TAGTTAACTTTCCAATCTGATTTATTCAGCTCTGCTCTATAACTATCAATTTCATCTGACATTTCATTAA TTATTGTTTGAATCTTAGCCCGATTGGTCACTAAATTCTTAATCAATTCTCCGCGGTGTTCATCAATTTC GTCTGTAACTTTTATACCATTGCCAAAGTCAATTTTGTCAACACTATCAGTTAGATTTTTGGGAATTTCC TTAAGTGCCTCCACATCACGTTTCAGTTCCCTATAAATATCTGTCTCTTTTGAATACTCATCATAAACAT CTTTATAACTCTTATGAGATAGCTTTTCTACTTGTTTTTTTAAATCTTCAATTTCAAGCTCTACCTTCGC CTTATCTTGTATTTCTTTTTTCACAGAAACAATATCCCAATTTAATTTTCTTATTTCGTTTACACATGTT TCAATCTCATTTTTATACTCTGAAATCAAATCAGAGTTATATCCATCTAGTAATTCTAGTATACTAGTTT GATTTTTTGAAATTTCATAAATTTGTTTTTGAGAGTATATACTCACCTTATCGGAAATCATTCTCAACCG TTCTGTTTCTTTTTCAACCAGACCATTCTCTTGTGTAAATTCATAAATAGAATGTCTATCCTGTGTTCTT ATTATTTGATATTTTGTCCCCTTATAGAAAATATTCAGTTTCACAATTGTATCCTTCAAAAATATTCCAG AGCCATTATGTTCAATTTGTGCAAAGTTCTGATAATCACTTTGTATTTCAGAAAATTGATCAAGTTCTGC TTCCTTTCCAAGGACATAGACCAAGAATCTTGTAATACAAGATTTGCCTGTTCCTCTTCCTCCAATAATT GAAGTTAAATTATCGCTGAATTCGACTACAACTTCTTTTGTGCTAAGAGTGGTCCCAGAAAAGCTTAATT TTTCCAATAAAACTTCATTTTCATTAATGGAGAAATTAGAAAAATCAGAATGAACTCGAGTTTCGCCTAG CATTAAAGCATCGCGAAGTGATCCCAAGTCAGGTTCGTCCTTCATTTTTATATAGGAATATCTTGTGCCT ATTCCCCAAAGTCCATGTTTTGAATTTCCTTTCGAGTGTGGGTTATCAGAAAAGCTAAGAAATGTCATAT TATTTTTTTCTGCCTCAACAACTGATTTATACCAAGATTCCAAAGTATCTTTTCCAACTCTACCATCATA TCTGGCATTCACACTATCATAGATAGTGTCACGCTTAGATGAGGGAATCATTTGTGAGCGATTTTCAAAA AATTCTTTTTGAACCGTCTGTACTGCATATATATTTTCTGATTCAAATATCTTTCCTAGATTTCCAAATT TTGCTGAAGAAATTCCATTAAACTCATCTATATGTGCAGGAATAACGAGCAAGCCCTCGTGCTCAGCAAA ATCAATAACTTCTAAAACTGATTTCATTGAGCAAGCTGCAGCGCTGGCCCAATCCTCTCTTTTTATACCC ATCTTGGTTAAGAAATCAGAGATATCATTTGTACATGCACTTTTATCAAATAGGACTAGTAAATGAGTTC CATTTTCTCCACACGTTAATTCCACTCCAGGAAAAAAATCTAAGCCATTTTCGATAGCCACTTTCTTATA TTCATCAATATTTTCCCCTGTATTGTGATCAGTTAATGCAACACAATCGAGTCCTTTGTTTAAACATTCT TCTACCCATTCCTTAGCAGTAACCTTTTTATCCTGAAAGCACTGACTCGCAGTTGAATGAATATGAAGAT CACATTTGTACCACTTATTGCCAACCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1786768-1788615	Sequence	HMPREF0351_11816 - metal-dependent phosphoesterase (Protein is coded in complementary strand; from 1788615 to 1786768)