Sequence retrieval

Genome: Cronobacter sakazakii ES15
Start position: 2540865
End position: 2541527
Length: 663

DNA sequence
>NC_017933, from 2540864 to 2541526 (663 bp); Cronobacter sakazakii ES15 TTACTGGGTTTGCTGAACGGCACCTTTACCTGACCAGATAATTTCGCCGGTCTGTACCAGCATCAGCTGC ATTTGCAGCGTAGGCGCATTCACATTACCGCTGGCGTTGGCATACAGCACATACTGCGCGCCCACGTTGC GGGCGATACCGATGGCTTTGCTGCGTGAACCCAGGCTGTCCTGCGGCGAAAGCCCCAGTTGCTGTTTCGC CATGGAGAGCTGCTGTGCGGAGACCAGCGTGAATTTACCGTTATTCGCCAGCGCGCCGCGCAGGGCTTCG GTGGCTGGGCCGGTTTGCATTGAACCGTTGGTGCGGTTGTTTACGCTATCGACCAGCAGCACGCTGCCTG CGTTCACCCCGTCAGCCTGCAACATTTTGCCGACCATCGGCGCCATTGCGCTGTTCCAGTCATAGGTACG CGCTTTTGGCTCTGGCGTCGCATTTTCAGGCTGATGTTCAATCGGGCCAGGCTGCGCCGGAATCGACGGC ACGGACGGCACGGTCGGCACGGTCGGTACCGGCTGCGTCGGCTGCGGCACCGGCTGCGTCGGCTGCTGCG TGCCTGGCTGCGCTTCTTCGACCGGTGCCGGTTTTTGCTCGCCGGGCAAAATACAGCCCGACAGCGCCAG GGCGACGGTCGCCAGCAGCGCATAGCGATACAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 2540069-2540887	Sequence	ES15_2380 thiK thiamine kinase (Protein is coded in complementary strand; from 2540887 to 2540069)
ORF.. 2540865-2541527	Sequence	ES15_2381 - hypothetical protein (Protein is coded in complementary strand; from 2541527 to 2540865)