Sequence retrieval

Genome: Streptococcus thermophilus MN-ZLW-002
Start position: 224621
End position: 226963
Length: 2343

DNA sequence
>NC_017927, from 224620 to 226962 (2343 bp); Streptococcus thermophilus MN-ZLW-002 TTACTCATCTACTGTAGAAGTAGAAGTAGAAGTAGAAGTGTTTAAATTTGAATTCTCAGCACCGTTAGAA CCCGATGTTGAAGGATTTGTTGATGCATCTGGGCCATACTGTTGACTATCTTGTGAACTTGATGAGTCAC TACTTTTACCATAATCCGAACTTGATGAGTCACTACTTTTACCATAATCCGAACTTGATGATGAGCTAGA TGTTCCTAGTGATCCGTTATAAACATTTGACGTTGACGAACCAGTTAGGAAGACGTAACTACCATTGTTG TAAACACCACTAGGCATATCGAAATCTTTCGAACCTGCACCATACATCTGGTTGAGGTATCTACTTGTTA GACCGTAGATTTGTTTAGCGATATTTAGGTCTGAGCCATAAAGTGGAGTTTTACGGTTTTCATAACCTGT CCAAATGGCCATTGCATATTTAGTATTATAACCAACGAACGTTTCGTCAGGAACAATAAGATCAGCGTAG ACGCCACTTTCTTTTTGTACTTGATAGTACTCATCGTCACTGTAGTTAGATGTACCCGTTTTACCGGCAT TGATCGTGCCAGGTACATAAGCTTCAGTCGCTGTACCACCTGTCAAAACCTGTTTCAACATGCTATTCAT CATATAAGCTGTTTGTGGCGACATGGCCTCGACACCTTTAGAATCGTATGATTTTGTTGAACCATCTTCA AATTTAATTGATTTAATGTAGGAAGGTTTATAGTAAGTACCACCATTGGCAAAGGATGCATAGGCAGCCG CCATCTTCTCACTTGAAGCACCATATTTAGGATCTGATGATGTTGTTGAAGATGAGATAGCGTTTGAATA AAACATCTCTGGGTACTCAATACCTAAATCTTTCAAAAAGGATTGAGCATAATCCAAGCCAGTCGCTTGA AGCGCTTTAACCGCAGTAACGTTACGTGATTGTTGAATCGCATAAGTCAGAGAGATACTTCCGTAATACT TGCGGTCCCAGTTGTAAACTGGAGTTGAAGTACCTGGGAAATTATAAGGGGTATCGTAAACGGTAGTACC AGTGTTAGTATATACCCTTTTTTCAATAGCAGGTGCGTAATCGGTGATAGGTTTCATTGTAGACCCCCAG TCACGATCAGTTTGAACAGCTTGGTTTGTACCAAGGGCAATGTTTTCGTCTTGGTGACGTGATCCCAATT GAGCAACAACACGTCCATTGGTAACATCAATGATAGTAGAAGCTACTTGAAATTTGTCATCAGGATAAGC AATATAATCATTTGAGTTGTAGACATTCCAAATGTATTTTTGAATATCAGGATCAAGATTTGTATAGACA TCGAGTCCAGCTGAGTAGATATCAGCATTGGCTTCGGTAGAGACTTGTTCAATAACTTGTTTGATGTAGT TATCTAGGTATGGTTCGTAGCTAGCTTTATTCGTAAGTGGAAGAAGACCATCTGATATATCCGTTGCTTT AGCTTGCACGTACTCCTCTTTAGAAATATTTTTGTCTTCATACATTTCATTAAGAACAGTGTTGCGACGT TCTTTAGCAGCATCCGGATTAGTATAAGGATCATATTGTGTTGGTGCTTGTGGGATACCAGCAAGAAGAG CTGCTTGGGCAACTGAGAGGTCACCTAATTCTTTACCAAAATAAGATTTAGCTGCGGTCTTCATACCGTA GTAACCATTACCCATATAAACCTTATTAACGTAGAAGGTCAAGATTTCTTGTTTAGTATACTGACGCTCC ATTTGGAGAGATAACCATATTTCTTGTGACTTACGTTTAAGGGTTTGGTCTGATGTATTAGTTGAGAAGT AGGCTAACTTAATCAACTGTTGGTCAAGTGTAGATCCCCCTTGAGTGCTGTTATGGCGAAGGTTGTTAAT AGCGGCTCCCATAATACGATAAACGTCAATGCCGCGATGGGTAAAGAAACGCTTATCCTCGATAGAGGTA ATTGCATTGACTAGGATTATAGGAATCTCATCTGTTGATGCACTTTCACGTTTTTCGGATCCCAAGTCAG CAATAAGACTACCATTACCATCGTAAATCCTACTTGAGTTTGTTGATTGCAACTTGTTCTCGGTAAGTTT AGGAACACTGCTAACATAGTAAGCAAAGAGTCCACCACCAGCGATGACACAGATTACAAAGAAAGTGAGT AGTCCAATAAAAGCGTATTTTGCAATACGCCATCCTAAATTTTGATCTGAAGCAGTTGAGCGTTTTCCTT TGCTATAGCGAGAAGAATCTGAACGTTTAACCTGTGTGTTTTTAGCACGTTTAGTTGAACCGTTAAGTGA TTCTTTAAAGTTGCTGAAATTAAATTTAGCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 224621-226963	Sequence	Y1U_C0220 - penicillin-binding protein 1A (Protein is coded in complementary strand; from 226963 to 224621)
ORF... 226963-227559	Sequence	Y1U_C0221 - Holliday junction-specific endonuclease (Protein is coded in complementary strand; from 227559 to 226963)