Sequence retrieval

Genome: Escherichia coli O55:H7 str. RM12579
Start position: 52160
End position: 54022
Length: 1863

DNA sequence
>NC_017656, from 52159 to 54021 (1863 bp); Escherichia coli O55:H7 str. RM12579 ATGGATAGCCATACGCTGGTTCAGGCGCTGATTTATCTCGGTTCGGCGGCGCTGATTGTACCCATTGCAG TACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCG ACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGCGTGGTGCTGATGCTGTTTATTATC GGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTTGGCGGTGGCGCATTGCAGA TGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCTGTATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATT GATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATG GTGACGCAAATGGGCCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCAATCCCGCTGGTGG CGATGATCCCGCTACTGGCGGCGAGCAGTGCCTCGACGACGATGGGCGCATTTGCTCTCTCGGCGTTAAA AGTGGCGGGCGCGCTGGTGCTGGTGGTACTGCTGGGGCGCTATGTCACGCGTCCGGCGCTGCGTTTTGTT GCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTTGTGTTTGGCTTTGGTTTGCTGC TGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATA CCGTCATGCGCTGGAAAGCGATATCGAACCCTTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTT GGCATGTCGATAGACTTTGGCACGCTGATTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCC TCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAGGTGCCAAATAAACAGCGTCGCTG GTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAAT GTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGGCGGCAACGCCGATTC TGCTGGTGATCCTCAATCGCCTTGAGCAATCTTCTACTGAAGAGGCGCGTGAAGCCGATGAGATCGACGA AGAACAGCCGCGGGTAATCATTGCCGGATTCGGTCGTTTCGGGCAGATCACCGGACGTTTACTGCTCTCC AGCGGGGTGAAAATGGTGGTGCTCGATCACGATCCGGACCATATCGAAACGCTGCGTAAATTTGGTATGA AAGTGTTTTATGGCGATGCCACGCGGATGGATTTACTGGAATCTGCGGGAGCGGCGAAAGCGGAAGTGCT GATTAACGCCATTGACGATCCGCAAACCAACCTGCAACTGACGGAGATGGTGAAAGAACATTTCCCGCAT TTGCAGATTATTGCCCGCGCCCGCGATGTCGATCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGC CAGAGCGTGAAACCTTTGAAGGTGCGCTGAAAACCGGGCGTCTGGCGCTGGAAAGTTTAGGTCTGGGGCC GTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATG GTTGAGAATGACACCAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATCA CCGAGGACCGCGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACAGAAGAAGGTAAACATAC CGGCAACATGGCGGATGAACCGGAAACGAAACCCTCATCCTAA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 51637-52167	Sequence	ECO55CA74_00230 - glutathione-regulated potassium-efflux system ancillary protein KefF
ORF.. 52160-54022	Sequence	ECO55CA74_00235 - glutathione-regulated potassium-efflux system protein KefC