Sequence retrieval

Genome: Escherichia coli O55:H7 str. RM12579
Start position: 5113565
End position: 5114737
Length: 1173

DNA sequence
>NC_017656, from 5113564 to 5114736 (1173 bp); Escherichia coli O55:H7 str. RM12579 TTATAAATGAATTTTCCTTTCTTTATGTAAAGGTCGGGTTACATAGAGATTTGAGAATCCAGGGAGGGGC TTGTGAAGAATGCTCTTACCATAGCTTCGGAGAAGCTCTGCAAGCCTTCTAAGAAATGGAAGACAGCCGA TAAGCCTGAGATGCTTTTGCTGGTTAACCGCATTCTTTTGGAGAATCTTCTCAACGATCTCGTTTCGTCT GTCTTTTCTGAGTCGCAGTTCGAGTTCAGCAATTTTTTCACTGATACTTTCCTTTCTCTCAGTGGTTTTT AAAATGGTTGCAGGAGCAGGAGCAGGAGCAGGAGCAGGAGCAGGAGCAGGAGCAGGAGCAGGAGCAGCAG GGGGGGCTTTTGTTGGTGTGTTGGTTCGTATCGCTTCTAAGTGGTCAGGGTTGTAGCTAAGTTGCTTTTG AAGATTTGCCCAAGAGTAACCCTTGCCTAACTGCGAAGCTTTGAAAGCTATATCGCGGTAAGAGAATGAG AAGCCGTTCATTTTACCGGTAGAGGCAATATTGGCCTTCCAGGATACTTCCGCTTCTTCCAGCCTCTCGA TAAAAGTTAAAAGGTCAGGTGTATCTGCCAAACTTTTATCAAGTATCTGCTGGAGAGCTTCTTTAGGGGA GGGAAGGACAGTCCGTTTTGAAAGCATCTGTTCATTACGGGAAACCTTTCTTCGCTTCGGTTGCTTTGGT GCAATGGCTGAGGCTGTTTTGGTCACTGTTAGATTATGAGTGATTTCAAGCTCACTGATGATCCGGGTGC TGATAAGGTTTTCGTTCCTACCCAAGTAGAGCTTCCCTCCATTGAGGTCTATGCGACTTGCGATAATGTG TATGTGCTGACCAGCCGGATCGTCATGAAGCACATAGCAACGAAGGTGAGTATCACTGAACCCCATCCTC TTCATGTAGTCATCTGCTATGTTTACCCATTGGTCATTTGATAATGTTTCTCCGTCAGGCAATCGTAGCG AGTTGTGCCAAACTGGTTTCTGAACATCGGGCCGAAGCTGTTTAGTGTCATCAAATTCAGTGATCAGTTT AGAGGCTGAATCACCTAACATGTTCCCGCCAATGACGACTGGATCGCTTTTATGATGTGCTCCCGGCTTC AATGCATATTGAACAACACCTGCGAAGCTTTTACCCCTCTTGATCTTCCGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 5113565-5114737	Sequence	ECO55CA74_24495 - hypothetical protein (Protein is coded in complementary strand; from 5114737 to 5113565)
ORF... 5114715-5115152	Sequence	ECO55CA74_24500 - hypothetical protein (Protein is coded in complementary strand; from 5115152 to 5114715)