Sequence retrieval

Genome: Escherichia coli O55:H7 str. RM12579
Start position: 3243480
End position: 3244760
Length: 1281

DNA sequence
>NC_017656, from 3243479 to 3244759 (1281 bp); Escherichia coli O55:H7 str. RM12579 TCACTTTTTAAAGAAAGTGATTATCTTTTTCAGCCTCCCCCAAACTTTACTGTTCATTTCTACAGTCTCA GTATTTTCCTTTAAAGAGGATCTTATTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTG GCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTC TGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCAAAGGAGAACTAAAATCT AATGAAGTTTCAATATTATATTCATGATTTTTATGGTCTATATCAGATAATGAAATGGGTTGTTCATTCA ATGATGATACAGACAGATCATTTTCAATGTAATAATTACTATCATTATATGAGCTTTCAAATTTACCCAA GAAAGCCACATATACTAATTTAAAGTGGTTTGTCTTAAATAGAAACTTATCATTTTCATCCGGCATCCCA AAAAAGAAAACCGTCAAGTATTTTTTAGCATTCCTTTTATCTTTAAGCCCCTTATAATACCTATCAGCTT CCTTCCTTTGCTTTTCATATTCCTTCCAAAACACATCCTTCTGACTTTTATTCCAGTCAGAAACATCAAT TTCAAGCGTAAATGGTTTTTGGGTTTTCTTATCCCTTGCGAGGAAAGTAATACAATGTTTATCTGAATCT TCATGCAGACGAATATTAGACTGGGCTACACCATAATAAATAGCAGGTTTGTAATAACGTATGTTGTTTT TGTAAAAAATAAGTTGAAATGTATCTTTATAAGATCGCGTGCCATATGGAGGGATTGATAGCTGTTCATT ATGATTGCTATTATTTATGTAATAGTCAACGACTGGCTTAATACTTGATGAAGATACAGGATGTTCATTA CCTTTTTTTTCATCCTTGTGATTTTCATTTTTAATATTTCTGGCATTTTTAACACCACCTCTTGGTTTCA CATCAATTTTTAAAAGTGATATAACCGGAGCAGGTGGCGAATTATGTTTTCTTTCGTCATTTGAAGTATG ATTGTTTTTATATCTTGGATCATGAATACCACATCCATCCTTATGTTTATCGCCATATCTGAAATATGAT TGATTAACATGTTTCATAGTAAAACTACAAGGTGTGGCTTTAATTTTACAAGGCTCGTATGGGCATTCAA AACTCGTTGTCTTAATGTAAAAATCATCCATTCCACGGAGATCATTTCCATGAATGATTTCATCAGTCGA ACTATCCTTCGCAATCGTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 3243480-3244760	Sequence	ECO55CA74_15665 - hypothetical protein (Protein is coded in complementary strand; from 3244760 to 3243480)