Sequence retrieval

Genome: Escherichia coli W
Start position: 74586
End position: 76196
Length: 1611

DNA sequence
>NC_017635, from 74585 to 76195 (1611 bp); Escherichia coli W TCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGC AGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCA GGGTACGGAAATCATCGTTACCGAACAACGCCACTACGCCAAAATCACCAATCGACAGCACGCAGGCAAA AGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCA ATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCA GCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGAT AGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCG CTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCA TCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA CAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGT GGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCG GGTCGCGCCAACCTTGCAGCAGCGTGGTGCCAGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCAC CAGACCGAGACAGCACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCGGGATCGTAGTCGTAACTC AGCGCCTGATAAATAGCCAGCTCGATAGTCGTCGCTTGCGGACCGCCGCCCAGCGACAACACGGTGGCGA AGCTGGCGAAACAGAGCATAAAGATAAGCGCGGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTC GACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCGGGGATG TTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCAGATTAAAAAATACGTGCGCCAGCAAAATAC CTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGCGATGCCAGCCAGCCCTG ACGACCATAGACGCTAAGAATGCCGAAAACAGCAACTAGCACCGGGAGGATCAAGGTCATTGCGCACAGA CGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGGGCGCGGGCGAGGAATATCGCGGGTACGA CAGAGAGTAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGGTAGCTGTCCTG CCAGACTGCCACCCAGTTACCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCGAGCGCAACCGCT ACCACCAGCGTGGCGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCA T

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 73904-74602	Sequence	ECW_m0064 thiQ thiamin transporter subunit (Protein is coded in complementary strand; from 74602 to 73904)
ORF.. 74586-76196	Sequence	ECW_m0065 thiP fused thiamin transporter subunits of ABC superfamily: membrane components (Protein is coded in complementary strand; from 76196 to 74586)
ORF... 76172-77155	Sequence	ECW_m0066 tbpA thiamin transporter subunit (Protein is coded in complementary strand; from 77155 to 76172)