Sequence retrieval

Genome: Escherichia coli W
Start position: 73904
End position: 74602
Length: 699

DNA sequence
>NC_017635, from 73903 to 74601 (699 bp); Escherichia coli W CTAACCCGTAATCCCCAATAGTGCCGAAGCTCTCGCCTTACCGCTCAACAACTCATCGGTCTTACCCTGC CAGGCGATACGCCCGTCGGCGACCACCACCGAGCGCGTGGCAATCCGCGCCGCATCTTCCACGCTGTGCG ACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGCAG CGCCGGATCGAGCGCAGAAAACGGTTCATCGAGCAATAAAATCGGTTGTTCGCGTACCAGACAACGCGCT AACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCA TCTGACGGGCAATCGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGCTTCAGTCCTGGATTAAGCCCCAG CCCGATGTTCTGCGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGG CGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAAC CGGCAATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCG TTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGATAAAGCCAGGTGATATCAGTCAGTTTTAACAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 73904-74602	Sequence	ECW_m0064 thiQ thiamin transporter subunit (Protein is coded in complementary strand; from 74602 to 73904)
ORF... 74586-76196	Sequence	ECW_m0065 thiP fused thiamin transporter subunits of ABC superfamily: membrane components (Protein is coded in complementary strand; from 76196 to 74586)