Sequence retrieval

Genome: Escherichia coli W
Start position: 3096156
End position: 3096383
Length: 228

DNA sequence
>NC_017635, from 3096155 to 3096382 (228 bp); Escherichia coli W TTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCT TTATACGAAATCATACCGGTATCGTTATCAGTCTGTGGTTTGCCGTCAGAGACGATGGTACGTCCGTCAT TGGTGTGCATCACATAGTTCGAACCGGAACAGGCGCTCAGGGCAAAAGTCAGCATACAGGCAGAAATAAT TGCGGCAGTCTTTTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 3096156-3096383	Sequence	ECW_m3020 ygdI hypothetical protein (Protein is coded in complementary strand; from 3096383 to 3096156)