Sequence retrieval

Genome: Escherichia coli W
Start position: 3095996
End position: 3096200
Length: 205

DNA sequence
>NC_017635, from 3095995 to 3096199 (205 bp); Escherichia coli W GGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTT TTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTT TTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF... 3096156-3096383	Sequence	ECW_m3020 ygdI hypothetical protein (Protein is coded in complementary strand; from 3096383 to 3096156)
3095934-3096139	Sequence	ECW_nc3019 -