Sequence retrieval

Genome: Escherichia coli W
Start position: 247968
End position: 250775
Length: 2808

DNA sequence
>NC_017635, from 247967 to 250774 (2808 bp); Escherichia coli W TCATAAAACCGTTTCATCCTTTGTGACGGACTCGTCGTTCTGAGCATCTTCCACGTCTGCCGTAAATGCG CCGTCCACTGCCGACAACCGAATCCGGGCAACCGCCGTATTCGCCGCCATTTTCTGCAACAGCAGCAGGG AGAGCGGCGGCAGCATATTGCCGTCGATGACCGATTCCAGCATCCTCGCGCCGTTTTCCGCGCGGGTGAC GCGGCTCATGATTTCGTCCGTCACTTCCGGTTCTATAATCACCTCCGCGCCAAAGCGGCTGCGCAGCACG TTATCCAGGCGGGCCAGTTTCCCGGCAATGATGGTGGCGAGCGTCTCTTTCGACAGCGGCAGGTACGGCA CCACTTCCATACGCGCCAGCAGGGCAGGTTTGAAGAAGTCGGCCAGCACCGGATACAGTACTTCCTGCAT GGTTTCCGGGTCATCGGCATGCTCGACTATTACCTGGTAGCCGAGGTTGGACGTCAGGAAGAAGACGATA TTTTTGCAGTCAATCAGGCGGCCTTCACCGTCTGCCATTTCGCCCTTATCGAACGCCTGGTAGAACAGGT TGAGCACATCCGGGTGCGCTTTTTCCACTTCATCGAGCAGTACTACCGAGTAGGGTTTCTGGCGAATCGC TTCGGTCAGTACGCCGCCTTCACCGTAGCCAACGTAGCCCGGAGGCGAACCAATCAGTCGCGAGACGGTA TGTTTCTCCTGGAACTCGGACATATTGATGGTGGTCAGGTACTGGCGACCGCCGTAGAGCAGCTCTGCCA GTTGCAGGACGGTTTCAGTTTTACCCACGCCGCTGGGGCCAGCCAGCAGGAACGCGCCCAGCGGGCGTCC CGGACGGCGCAGGTCGGCGCGTGCGGTCAGCAGGTGTTTGTGCAGGCTGGCAATCGCCAGGTCCTGGCCT TTGATGGTGTCGCCGAGCCATTTTGGCAGGTCGGTGATGACCGACATTTCATTCTGCGACAGGCGGTTAA GCGGCACGCCGGTCCATTCGGCAATCACCGCCGCAATCTGTTTTTTATCGACGTGCGGGGAAACCAGCAA CCGGTCGTTATGCAGGGCGTCCAGTTCGGCAGTGAGATGTGCCAGACGTTGTACCGGGGAAACTGTTTCA GCGGTCTCTTCCGGCTGTTCGCTGCCAGCTTCATCAGCCGGTTCGGCACCGGTATTATCCTGCTCTGACT CTGACTCTGACTCTGACTCTGACTCTGACTCTGACTCTGACTCTGACTCTGGCTGCGTATCCTCCACGGT ATCTGCGTCCGGCAACGGCGCCGCATCGTCCTCTGCCACGCCCAGTAACTGCTGGCGCAGCGCAATAATC TCCCGGACCAGCGTCTGCTGCTGGTGCCAGGCCGCTTCCAGTTCATCCAGCGCCGTCAGCGTTTCATCAT ACTGCACCAGCACCTCGGTCATCCGTGATGTGTCGGTACGCAGTCCGATGCGAAGCTCGCGCTCAAGCTG GCGAATTTCCGCCTCCTGCTGGTGGCTCAGAGTGGTCAGCGCCGAGATTTGCTTCGGCGGCGACGACAGG TTGATGGCCACACGGGCGCAGGCGGTATCCAGCACATCAATCGCTTTGTCCGGTAACTGACGCCCGGAGA GATAACGCTCGCTTAATGTCGCAGCGGCCTGCAAGGCGTCATCATCAATCAGCACGCCGTGAGACTGTTC ATAGACCGCCGACAGACCGCGCAGAATAATGGTGGCTTCGGCAGCGTTGGGTTCGCTGACCTTCACCAAC TGGAAGCGGCGCGACAGGGCGGCATCTTTTTCGAAGTATTTTTTGTACTCGCTCCAGGTGGTGGCGGCGA TGGTTTTCAGCTCGCCGCGCGCCAGCGCCGGTTTGAGCAGGTTGGAGATATCCAGCCCGCCCTGCTGGTT GCCCGCGCCAATCAGGGTATGTGCTTCGTCGATAAACAGAATGACCGGCACCGGGGAGGAAATGACCTCC GCCATCAGCCCTTTGAAACGTTTTTCGAATTCACCCTTCACCGACGCTCCGGCCTGCAATGCGCCCAAGT CAAGGGTCATGATATCGGTGTTTTTCAGCTTGTCCGGCACATGGCCTGCCACGATGCGCAGCGCCAGCCC TTCGATTAAGGCGCTTTTACCCACGCCTGCTTCGCCCACCACCACCGGGTTGTTCTTGCGGCGGCGGCAG AGAATGTCGATCATCAGGTCGATTTCGTGGTCGCGGCACAGTACCGGGTCAAGCCTGCCGTTACGGGCGT CTGCGGTCATGTTTTTGGCGTAGCGGGCAAGCAGGGTGTCAGCAGCGTCCGTCAGTGTGCCTGCGCCTTT ACCGTCTGCATCCGGCACGACTGATTCCGCCGACTCCTGTGTCCATTGCACAAAGTCCTGTTGCAGACGG TCACGGTTAATGCCGGTCAACAGCCGGGCGGCAGCAGGCGGTATATAACGCAGCGGCGAATGCAGCAGGG CCAGCAGCAACACGCCGCCACGCAATTCGCTGTGCTGCATCTCAGCCGACGCCAGCAGCCAGCCCTCTTT CAGCCACTCAACCAGCATCGGGGAAAATGCCGGGTAGCTGTCCGCAGAACGGGCAGTTGTGTAGTTCTCC ACCGTCAGCGCCTGGCGCAACTCTTCCATGCCAATATCGCCCTGGCGGGTAATAACCCGCAGGTCACTGC GTGGCATGGCGAGCATCTGAATAAGCACATGGCTGACGGTAATTTCCGCTGCCTGCTGGCTCATACATTC AGAGGCGGCCATCTCCAGCGCCTGGCGGGAGAACAGGTTAAGCCGTTTTACCAGCGTGGGAAGATCAATC TGGATCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 247228-247971	Sequence	ECW_m0221 etsX T6SS-associated protein (Protein is coded in complementary strand; from 247971 to 247228)
ORF.. 247968-250775	Sequence	ECW_m0222 etsV ClpV ATPase (Protein is coded in complementary strand; from 250775 to 247968)