Sequence retrieval

Genome: Escherichia coli W
Start position: 1166665
End position: 1169211
Length: 2547

DNA sequence
>NC_017635, from 1166664 to 1169210 (2547 bp); Escherichia coli W ATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCG CCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGCCGTGCGACTGCGGCGCAAGCGGCGACTGAGGCTGT CATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCCGCGCGACGGTGAAGGATGGTCGC TTTGTGGCGGCAAAACCGTTCGAACTGGATAAATATCCGTCGAAAATGATTGCCGGATTGCCGGATCACG TACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGA TACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAA GAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGG GGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGG CGGAGATTACTCTACCGGTGCTGCGCAGGTGATCCTGCCGCGCGTGGTTGGCTCAATGGAAGTGTATGAA CAGCAAACCTCCTGGCCGCTGGTATTGCAGAACAGCAAAACCATTGTGCTGTGGGGCTCTGATTTGCTGA AAAACCAGCAAGCGAACTGGTGGTGCCCGGATCACGATGTTTATGAATATTACGCGCAGCTAAAAGCGAA AGTCGCCGCCGGTGAAATTGAGGTCATCAGCATCGATCCGGTTGTCACATCCACCCATGAGTATCTGGGG CGCGAGCATGTGAAGCACATTGCGGTTAACCCGCAAACTGATGTGCCGCTGCAACTGGCGCTGGCGTATA CGCTGTACAGTGAAAACCTGTACGACAAAAACTTCCTCGCTAACTACTGTGTGGGTTTTGAGCAGTTCCT GCCGTACCTGCTGGGTGAGAAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATT GATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAACGCAAATTATTGCTGGCTGGT GCGTGCAGCGTATGCAGCACGGTGAACAGTGGGCGTGGATGATTGTGGTTCTGGCGGCGATGCTGGGGCA AATTGGCCTGCCAGGTGGTGGCTTTGGTTTTGGCTGGCACTATAACGGCGCAGGCACGCCGGGGCGTAAA GGCGTTATTCTGAGTGGTTTCTCCGGCTCTACGTCGATTCCGCCTGTTCACGACAACAGTGATTACAAAG GCTACAGCAGCACCATTCCGATTGCCCGTTTTATCGATGCGATCCTCGAACCGGGGAAAGTGATCAACTG GAACGGTAAATCGGTAAAACTGCCGCCGCTGAAAATGTGTATTTTTGCCGGAACTAACCCATTCCATCGC CATCAGCAGATCAACCGCATTATTGAAGGCTGGCGCAAGCTGGAAACGGTTATCGCCATAGATAACCAGT GGACCTCAACCTGCCGCTTTGCCGATATCGTGCTGCCTGCGACCACGCAGTTTGAGCGTAACGATCTCGA CCAGTACGGCAACCACTCCAACCGTGGCATTATCGCCATGAAACAGGTGGTGCCGCCGCAGTTCGAGGCG CGCAACGACTTTGATATTTTCCGCGAGCTGTGCCGTCGCTTTAATCGCGAAGAAGCCTTTACCGAAGGGC TGGACGAAATGGGCTGGCTGAAACGCATCTGGCAGGAAGGTGTACAACAAGGCAAAGGACGCGGCGTTCA TCTGCCAGCGTTTGATGACTTCTGGAATAACAAAGAGTACGTCGAGTTTGACCATCCGCAGATGTTTGTT CGCCACCAGGCATTCCGCGAAGATCCGGATCTCGAACCGCTGGGCACGCCGAGTGGCCTGATTGAGATCT ACTCGAAAACCATCGCCGATATGAACTACGACGATTGTCAGGGGCATCCGATGTGGTTTGAGAAAATCGA ACGCTCCCACGGTGGGCCCGGCTCGCAGAAGTATCCGTTGCATCTGCAATCTGTGCATCCGGATTTCCGA CTTCACTCGCAGTTATGTGAGTCGGAAACTCTGCGTCAGCAATATACGGTAGCGGGTAAAGAGCCAGTGT TCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCG CGGTCAGGTGTTGGCAGGGGCAGTAGTTTCTGACCGCTATGCACCCGGCGTGGCACGAATTCACGAAGGG GCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCACTGTGCAAATACGGTAACCCCAACGTGCTGA CCATCGACATCGGTACTTCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAA GTACAATGGAGCAGTGGAGCAGGTGACGGCGTTTAACGGCCCTGTGGAGATGGTGGCGCAGTGCGAATAT GTTCCCGCGTCGCAGGTGAAATCATGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1165493-1166665	Sequence	ECW_m1106 torC trimethylamine N-oxide (TMAO) reductase I, cytochrome c-type subunit
ORF.. 1166665-1169211	Sequence	ECW_m1107 torA trimethylamine N-oxide (TMAO) reductase I, catalytic subunit
ORF... 1169208-1169807	Sequence	ECW_m1108 torD chaperone involved in maturation of TorA subunit of trimethylamine N-oxide reductase system I