Sequence retrieval

Genome: Escherichia coli UM146
Start position: 4837332
End position: 4838942
Length: 1611

DNA sequence
>NC_017632, from 4837331 to 4838941 (1611 bp); Escherichia coli UM146 TCAGTCAGTTTTAACATTTCGCCCCGGTATTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGC AGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCA GGGTACGGAAATCATCGTTACCGAACAATGCCACCACGCCAAAATCACCAATCGACAGCACACAAGCAAA GGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCA ATGCCCAGCGACTGACACAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCA GCGCATAAGGGATTGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGAT AGTGTTGTTGAGCAGTAAAAAGAAGCCCGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCG CTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCA TCATGGTCAGCACTACGCACAATACGCCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA CAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGT GGCAGCAACAGCAGCAGCGCCAGCACAATTAACGCCGTGTCGCAAATGCGACTATGCAGACGATCGTCCG GGTCGCGCCAGCCTTGCAGCAGTGTGGTACCAGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCAC CAGCCCGAGGCAGCACACCATCTGGATCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACTC AGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGA AGCTGGCGAAACAGAGCATAAAGATTAGTGCGGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTC GACGAAACGGAAAAAATGCCAGCCGTGCATCCCAAGTTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATG TTTTCCAGTGCCTGGAGTAATAAACGGCTAGCCATCGGCAGATTAAAAAACACATGGGCCAGCAAAATGC CTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCAAGTGATTGCCAGAGCGAGGCCAGCCAGCCCTG GCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATCAAGGTCATTGCGCACAGA CGCAACAGCATCTGTCGCCCAGGAAAGCGCCTGCGATAGAGGGCGCGGGCGAGGAATATTGCGGGTACGA CAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGGTAGCTGTCCTG CCAGACTGCCACCCAGTCACCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGTGCAACCGCC ACCACCAGCGTGGCGGCGCTTACGCCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCA T

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 4836650-4837348	Sequence	UM146_23125 thiQ thiamine transporter ATP-binding subunit (Protein is coded in complementary strand; from 4837348 to 4836650)
ORF.. 4837332-4838942	Sequence	UM146_23130 thiP thiamine transporter membrane protein (Protein is coded in complementary strand; from 4838942 to 4837332)
ORF... 4838918-4839901	Sequence	UM146_23135 tbpA thiamine transporter substrate binding subunit (Protein is coded in complementary strand; from 4839901 to 4838918)