Sequence retrieval

Genome: Escherichia coli UM146
Start position: 4524989
End position: 4526362
Length: 1374

DNA sequence
>NC_017632, from 4524988 to 4526361 (1374 bp); Escherichia coli UM146 TTATCTTCTGTCCGGAAGAATCAGAGGCTGCGGTTTCAGACTGTCTGCCGGTACATTCCTCTCCCCGTTA AAAACCATAACGGGTTCATTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACGAATGC CGGTCAGCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTGCTGACGGACTGATGAGACGT GATGTCACTGGCGGTAATAATCAGGGGAACGCTGTAGCCTCCCTGCACATGACCATCATGATGAACAGGA TTAGCACTGTCGCTGACCGACAGACCATGGTCAGAAAAGTAAAGCATGGCAAAATGACGGGAATGCCGGC GAAGGATACCATCAAGCTGACCGAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAACCTT TCGGGGATACTGCTCCAGGTAATGATTCGGCCAGGAGTGAAGCCGGTCACACGGGTTTGGATGAGACCCC ATCATGTGCAGGAAAATCACCTTCGGAGAGGATTTATCCGCCAGTGCACGTTCTGTTTCCTGTAACAACA ACATGTCATCCGTTTTACGGGAAGCAAAGCTGCCTTTCTTGAGGAAAACGGTATGCTCCGCATCAGAAGC AATAACAGAGATGCGTGTATCATGCTCCCCCAGTTTTCCCTGATTGGATATCCACCATGTGCTGTATCCT GCTTTTGCTGCCAGCGCCACCACGTTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCC GGCTCAGGGAAGGCACAGTACTGGCTGCTGCCGATGTATAACCGTCAATAAATAAACCGGGAGCCGTATT CAGCCACGGTGTGGTTGGCACAGGATAGCCATATACCGACATATAATCCCTGCGCACACTCTCACCAGTG ACGATAACAATCGTGTCATACAACGGTACACCCGGCAGGATTTTCCAGTTGTCAGCCCCGTGCTGATTCA GTTGTTTATAACGCTGCATTTCACGCAATGTGTCAGTTGTCCCCACAACAATTCCTTTAACCATCCGCAA CGGCCAGCTGTTTACTGAGCATAATACGAACAGCAGCAGTGCCAGCCAGTTACGGTGACCGCGGTGGTGT GTTCGCCAGAAAATCACCATGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAATCATCC CGGTAAACTCCGCTGCCTCATCAGTTGTGGTCTGCAACAACGCGACAATAAAACTGTTGTTGATTTTACC GTACGTCATACCGGCAGGCGCATACAGTGCACAACAGAACAGAAATAACAGCGCTGTAATGGATGTGAGG GTATTTCTGTGTGCAAGGAGCAGAAGGAGAAACAGAAGCAGCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 4524989-4526362	Sequence	UM146_21620 - hypothetical protein (Protein is coded in complementary strand; from 4526362 to 4524989)