Sequence retrieval

Genome: Escherichia coli UM146
Start position: 3422245
End position: 3422832
Length: 588

DNA sequence
>NC_017632, from 3422244 to 3422831 (588 bp); Escherichia coli UM146 ATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAAGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTA GCGTGCTGGCGTTTATGATTTTTTCTCAAGCTTCCGAGTTGCATCAACGTTCACAGAAAGAAATCCAGCA GTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGCGGAAT CGGAGTACGGATAAAATCATGGTGTTGGGTGAAGAAGCGATTTTTACCACGCAAAGTCGCGATCCGCTGG CCCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGC TGTTCGTACTTCTGTGGATGGCAGGAAGGATCAACCCGCCCAGGCGATGCTGGAACATGTCGAGAGCTTT CTTCTGGAAAGTAACAGCGGAGAAAGCCAGGAGCTTCCTCTCTCAGTCACGTTACATCTGAAGACTCAGC AATATGGGGCGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGAC GCAGGCCGGGAATAATAACCATGAATAA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 3421839-3422252	Sequence	UM146_16560 - putative general secretion pathway protein I precursor
ORF.. 3422245-3422832	Sequence	UM146_16565 - putative general secretory pathway component, cryptic
ORF... 3422825-3423808	Sequence	UM146_16570 - general secretion pathway protein K