Sequence retrieval

Genome: Escherichia coli UM146
Start position: 1602309
End position: 1603967
Length: 1659

DNA sequence
>NC_017632, from 1602308 to 1603966 (1659 bp); Escherichia coli UM146 TTACTCATGATCGTTATTTATCCACTGGCGAATGACCAGCGCCACCACGCGCGGATCGTTATCAGACATT TCACGGATACGCTGGCTCATGACTTCTGCCCCCAGTCGTTGGTTAGCGCGCCGTTGTTGGAGTTGTTCGT CTTTGCTCAGACGGACTTCCACCGCATCTTCCACTTCCTCGCGGGCCTGCGCCTGTTGCTGCACAGTCTT CACCGCCTCGGCGCGACGTGTTAGCTGCGGGCGTACCGCTTTCCGCCACAGCAACCACGCCACCAGCAGT ACCAGCAACCAGCGACCGGCAGCCAGCAACTGATCGATAAACACTTGCTGTTGCCAGAACGGCAGTGCTC CGCCGCTTTCGTCACTGCTATTGAACGGCGAGTTAACAACATTGAGCGAATCACCGCGTTTTTCAGAAAA GCCCATCGCCTCGCGGGTCAGAGCTTCAATTTGCTTCATCTGTTCGTTGCTGAGAGGCAATGGTTTACCG TCTGGCAAGGTTTTGTAATTCACCACTACCGCGACTGACAGACGTTGCACATCGCCCACGTTCATTTTGG TATGACGAATGGTGCGATCGACTTCGTAGTTACTGGTTTCATTCCGCTGTGTGCTACGCGGCCCACTATT GCTGGTGGTGCTCGCCTGCTGCTGGCGGTTATTTTGATTTGCCGGAGGCGTGCTGATTGGCGCGTTATTC GCAGGTGCCGGTTGATTCGACAACGCTCCCGGTACGCCGCCCGGATAACCGGAACCGCTTTGCTCGCTCT CATTAAGCTGGCGTGAACGAAGCGCCGCATGAGATTCATCACCGTTAGGGCGATACTGTTCTTCCGTTTG TTCTTTACTGGCGAAGTCCAGTTGCGCCGTGACCTGGGCGTGAATATTACCGTTACCAACAATAGGCGAC AGGATCGCTTCAATACGCCGCTGAATACGGCCTTCGACATCGCTGGCATATTTCAACTGAGCGTCATTAA GATCGCGCCCGCTGGTATTGGACTGGGTTAACAGATGCCCGCCCTGATCCACCAGCGTGACGTTTCCTGG CGGCAGACCAGCAACCGCGCTGGAAACCAGATGTACAATAGCGCTAATTTGCCCTTCATCGAGTGCGCGG CCGGGTAACAGATTTACCGTCACCGATGCAGAAGGGGATTTTTGTTCACGGACGAATAAAGACGGTTTCG GCATTGCCAGATGTACGCGCGCCCCTTTTACCGGGCCGATAGTTTCGATGGTACGAGAAAGCTCGCCTTC CAGCGCCCGCTGATAATTCACCTGTTCGCTGAACTGGCTGATACCAAACTTTTCCTGATCGAGCAGTTCG AAACCGACCGCGCCGCCTTTGGGCAAACCTTGTTGCGCCAGGCGCAGGCGCAGTTCGTGAACTTTATCTG CCGGAACCTCAATAGCGCCGCTGGCTTCGCTGAAGCGGTAAGGAATATTCATTTGCGTCAGTTGGCTGAC AATTGCGCCACCATCCTGATCGGAAAGATTGCTGAATAATGTGCGGTAGTCGGGGGCTTTCGCCCACAGG ATCAGTGCGACCATGACCGCCACTGCTGCGGAACCGGCAACAATCAATGGAATTTTCGGATTCGCACGCA GGCGATTAAGCCACTCAAGAGATTTTGTCTGGGCTGCAGTCGCATTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1601321-1602316	Sequence	UM146_07490 fliG flagellar motor switch protein G (Protein is coded in complementary strand; from 1602316 to 1601321)
ORF.. 1602309-1603967	Sequence	UM146_07495 fliF flagellar MS-ring protein (Protein is coded in complementary strand; from 1603967 to 1602309)