Sequence retrieval

Genome: Escherichia coli UM146
Start position: 1007887
End position: 1008492
Length: 606

DNA sequence
>NC_017632, from 1007886 to 1008491 (606 bp); Escherichia coli UM146 TTAAGCAGTTGCCCCTTCTAATTCGTCTCGTCTGATGTTGTAAACGTCCTGCGCTTTGTGCTGCTCCGGC GTCCCTTCGAGCATCTTCCACGCTTTGGCGAACGCCTGTTTAAGCTCTTCTACGGTGTTTTTCTGCATTG CTGCGTCAGTGAATGCTTTTAGAACCTGTTCAGGTGTAAGTGATGGTTTTGATTGCTTTGCTGCTGCGTT CTGCTGATGTTTATGCTCGTCTGTATCTGCATCTTTCGCATCATCAATGCCGAACAAACCATTGAGGCAA TACTTGCGTGCATAAGAGCTTGTAGCTCCCGTAACTTGTGCAGAATCCATTCCTTTCTTGCTTTCTTCCT CTCGTGCAAGAGCGGTTGCCGTATGACTGTTTTCGCCATCGGTAATAGTTGCCGTGGCTTTCACGTAATA CCGATCACCAATCAACACAACTTCATCGCTGATTGATAAAAACAGACCGTTCAGTAACGGCTTAACGCCT TCAAGAATATCTTCGCAGCTTCTGTATTTATATTTGCCGAATGAGTTGTACTGATTCTTTGGCGCGTTCA GATTCTCCTGAATAGCTGCCAGTCTTGCGTAAAATTCTTTGCTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1007504-1007887	Sequence	UM146_04815 - hypothetical protein (Protein is coded in complementary strand; from 1007887 to 1007504)
ORF.. 1007887-1008492	Sequence	UM146_04820 - DNA single-strand annealing protein; essential recombination function protein Erf (Protein is coded in complementary strand; from 1008492 to 1007887)