Sequence retrieval

Genome: Erwinia sp. Ejp617
Start position: 814447
End position: 818910
Length: 4464

DNA sequence
>NC_017445, from 814446 to 818909 (4464 bp); Erwinia sp. Ejp617 TCAGCCTTGCGACGCGGTCCCGGAGACTGAATCAGAGAAACTAATCTGACTTTCTGCTTTGTCTAAAAGA TTGCTGACGTCCTTCCAGCGAAACGTAGCGTATTTACACGCGAGATACTGGATAAACAAAGCTAAAAAGT CGGCAGTGCAAGTTATCCGGTCAGGGATTGTAATACCGTGACGGTGCATTGCGTCAATAATACGATGATC CGAAGCTTTCAGGTTCAGAGTTTCGTCCATTCCCAGTTCAAATAGTAAATCTTCCCCTACTGTCATAGAG TCAAGACCACGTTGTTTGTTTATTTCCTCTATGAAAGATATGTAATTACTCATCACATTCACGCCTGCTT TCTGACTTTTCTCTTTTATTGTTGATGCACCAGCTCTAGGGCGGGAATGATCAGGAAAATAAAAATAATC GCGTATTTTTTTGTCTGCTGCGTGTGCAGATTCATGCAGAAAAGACCTTTCAATAACCCCTTGATTCTGT TTGATAAAACCAAGGTCACTTATAAACATCGCTTTGGTGTCTAGCATATAAGCTGCGATAACTCCAGATT TGTATACAGCGGATCCTTCTGGCAAAGAGCCGAAAACAATATGAGACGATTCTGTAGCCCGATAAATCTT AAAGGCGTCTATTGTCTTTAATACATTTTGTTTTACAATCTCGGCTTGCTTAACGGTTTCGAGTCGGAAT GTTGTAAAACCTTCGAAAGAGGTCATTATTTCTGACAGCTCCATTTCCTTTATGACTTTTTTAACCTCGC TCCCTATTTCGAATACGTTATCTAAGCTGGCTTGCAGTATTTTATACCGAACAGGATTAACTCTCTTTAA AAGAGGCAACTTATAAGCAGAGGGTATAATGTCATTATTTAATTTCATTTCAAAAGGCGTGTTCACACCT TGACCTTCATAAGGTCTCGCCTGGCACAGTGCATCGAGAGTTCGTTTGACGCGCGCGCCCGTGGGGCACA GCGAAGTGATAACCTCTTCCTTAGAAGGGCCTGCCGCAGCATCGACTTTCTTTTCTTCCTTCCGGGGAAG CGCTAATTTATGATCGCTGATATCATTTTTAGGTGACCAGTTATCCGGGCGTTGACGTACTAACTTCAGC TCCCCTTTCTCTATCAGATAAATCTCTTCCAGAAACTCCATCGTTTCAGGATCAACGATCGCATAATAGT TCTTTCCGCCCGCTTCACCCACGATATTGACCGGCACTTTAATGTTTGTCCCCGGAAGATTGGCTGTTTC AGTCAGTAATGCTTCATGAGAAATGACCTGTTCTGTTGCGCCGGGCTTTTCAATTTTTTTAAGGGCTTTA GTCAGTACTGTATTCAGCTTTATTGTATCTTCAGCACTCACCCCCACCCGCAGCTTATTGAATAGTGTTT TTAATCCCGCTCCAGAGTATTTGCCCACGGCCGTTATCAGCTCAAAACCTGGATCCAGCGTCCGAATGGT CGCTTTGCTCAATACGGCCAGCTCAGGAATGGTTGGCAAACTGATTTGTGCCAGCGCCTGCCGGCCGACC GTGTTTAAGATCTTCGGCGACACGGCTGCTGCAGGTAAAAGTTTTATCCCCACTTTCGCCGCTCTGTAAA GACTGAATCCAAACTTCCCCGCCAGGTTTACAGACAGGCCGACCACAGGGATAACGGAAACCACGTCGAT AATACAGGAAACGATATTGCCGTTGAAACAGCCATAAAAAGGAATAATACTCTTTAAGAATTCAATGCCT GTTTGCTCCACCGTCATATCATAACCTGACAACAGCAGCTGACGGTAAAGCTCCTCACCGTGTGTTTTCA CAAAATAAGCAACGACATCGTCTGCCGCATTCTTGTTGACGTCAATAACCGATTTAAACCCTGTTAATTC CACTTTGTACGCCTGCCGGGACTGGTCTCCCGGGGAGTCAAAAATGCCATACTCGAAATAATTATCAATT TTTCTGTCGAGACGACTGACAGCATATTCGCCGTCCCCGTTCCTGACAAATGCATAAATTCGCCTTTCCT TACCGACAACCACGCGGAAAATGTTCTTGTTTTTCAGTGCGGGTTTCCATGATTTGAGTGGAGACGTGGG GTAAACAGAGGGTGTGACATGCCTGATAACAGCACTACCTGAATTGATAAAGTCCAGTTCTTTTCTGTCA AGCCCGCCCATTGCCACAGCCAGCACAGACTCCTCCACGGCAGAAAATGCATTCGCCAGGTCCCGGGTTT TGCGTTCGTACTCTGTGGTAACGCTGGCATCCGGGCATCCCCGGGGCGTCTCGCCATTGAGATAGCGTTG CCGTGCAACGCCCTTGCCCAATCTGATTAATTCAGCAGGGTCCACAACCTTCGTCCCGGCCGGAACCCTC CCCCCGGGCACATATCCTGGATTGACCGTTTTTCCCAGTCCAATCAGCACGCTCTCCGGGCATTTCTCTA CATATCTTTCCGCTATTTTTCCACGGGATTCCCAGTCATCTGAAGCCTCCCCGAATGTCTTAACCCTTGC GCTCACGGTTTTATAAACAGGGGCGACCATACAACGAAATGTGACGTACTCGCTCACCGCCTTCAGCCGG TGAGCGAGAGTCTCTTTCTTTCGGTGAGAAACCCGTTTCGGATGGCTCACCGCCTCAAACAGCAGGGCCG GCAGGGCGAGGTATCTCAGATAACTTATGTCCATATCTCCGGCTTCCACCCTCTGCCAGAGCGCTTTCCC CACCGCCTTGCACTCTTCCACGCTGAAGTCACTCAGTGTCGCCCCCGCGTCTTTCAGCAGGAGCGAACCG GTATGAAGCCAGACAAAATTGTCTTCCGTCACGCGCGGCGATACTCCCCTGTTCGTAAAGGACAGCGTGT TGTCCACCCCATAATTCCAGAACTTTTCAAACTGCAATTTTTTTCCTTCGCTCAGCCCCTCCGTGTCAAT TATCCCGTCCGAGCGCAGTGCGTCAAACTTCAGCAGGCGTTGCAGCAGCAAGGCGTTTGCATCACCGGGG GACTTAATAACGGCCACTCTACTGGCAAGCCAGGACTCGACCGGCATCCCCAGCACATTATCAAACAGCC AGTTTCGCACCACCATGCCGGCCCGTCCGGCGCTGATTTTTTCGTCTGCCCGGCCGTGATAAAGCTTTTC AGCCTGCAGGATCATTTTTGCCACCTCTTCCAGCCTTTTATCAAGCCGGGTCACGTCATACATAATATCC GGATTGGGGGCAAAAAGCGTGATGCCGACGGCGGCAATGATTTCCTCCTTTCTAAAGGAGGCGGGGTTTT TCTCTTCATCAAGAATACCGGCCGACTGCATTATCCCGGCCAGCCCTGTATTGCTTGTCTCCGCTTTTGT CGGTTGATGCTGCCTGTCGTCCTTGAGCGCCTCCGCTGATGATGCCTCCGCTAATGGCTCCTCTGCTGAT GGCGCAGCCTCATCTCCCGCAGGTCGCGGAAGTAACCAAGATTGCAACCGCTTGTAGATCCCGGTGGATA CCCCGACAATCACGGTGCCCGTGCCGACAATCACGGTGCCCGCGACGACAAACAGCCCCGCCGGAGTCTT CAGCGCCCGAGTCAAGGCAGGCAGCACTCCCCCCGTGGCTAAGGCGGCTGCCCCCCCGACGTTCGCGTAA TTATAATCATACCGTTGTGCCGCATCATTCGCCTGCGGTGGCGGTTCATCAGCCCGCAGCGGCGGTGTCG GTTCATCAGCCCGCGACGGCAAAACCGGGACGGGGGCGGGCAGAGGACCCTGACGTGCCGTGGCATCACA TTTCGTATTGAGATGGGTATGGGAGATTTCCGTCCCGCGACTCTGCTCCTTCGTTTTCGTTTCAAGGGTT TCGCAATTGTGCAGGCCGGACAGCCCGCTTTCTCTCATCAGCTTCGCCATCGTCCTGGCGTGTGCTTGCT CCGCCAGGCTGTGGACGACTTCAGGGTTCGCCGTCATCTGGTTTTCCACCGTTGCCCTGGTATAAGCTTC AGGGTTCGCCGTTGAGTTCGATGAAAAATAGATTACGTTGACTTTCTGCACGCCGCCGGCCACGCCGGAA CCCGTCGCTTCGGCGCCGCGTAAGGTCGTTGCAATGCTGCTGTCCACCTCGAACGCCGGCGGCCGGGCGG GTTCCTCCGGGGGCGGGGCGTTATGCACCATCGCGTTCAACCCTTTCCAGTACGTATGAGCGCGTAGCAG CAGATTGGCTGCGCAGGCAGGCACCCGGAATAAGGCGCGCTGCGGGGCATCCGCACCGGTTATAAAGTGG CTGGCGACTACGGCCGACACGGCCAGAGCCATATACAGGTGCTCCTGATGGCCGTCATCCTCGCCCGGAC CGTCGCGCAAAAAAGACGCCCCCGTCCAGCTGATTATCGTATCCCTGATAAATGCCGCCAGCCTGGAGAT GATATTCATATCATCAGGAAGGCACCAGGATGCCAGAGAAAGGGTGTTCAGCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 814447-818910	Sequence	EJP617_07540 - hypothetical protein (Protein is coded in complementary strand; from 818910 to 814447)