Sequence retrieval

Genome: Erwinia sp. Ejp617
Start position: 475138
End position: 476469
Length: 1332

DNA sequence
>NC_017445, from 475137 to 476468 (1332 bp); Erwinia sp. Ejp617 TCAGGCCATACGCGCAATCTGCCCGGCCAGCCCGGGCAGCTGCTGCTGCATCTCCGCCGCCAGCTGCCTG AAACCCGCACTGAAAGCGCCCTCTGCGCTGATTAACTGCCAGTATTGCCCGGCCATTTCCAGCGCGGTTT GCCAGTGGTGCTGGCTCACGCTGTCATCAAGCCGTAGCTCCACGGCGCTGTCGCGCATATAGCCCGCGCT GTTCGGTGCGAAGCTCATCGGCAACATGTCATAGGCTGGCGTCAGTCGTAAGCCGCCTTCGGTGAGGAAA AACGACAGGTTGCCGGCGTGCATATCGCCATTGGCAATCAGGCGACCAAATGCCCACTGTAACGTCCCGC GCTGTGCATCGCGCTCGCTCAGGCGCTTCTGCTGTACCAGCTGCCGTAACGCAGCAGGCCAGTGGTGTGA ATTGCCGATAAACTCTGCCTGCACCGCCTCAAGCGAGACCATGCTGCGCCGCCCGTTTTCCCCTGTGCGG TCAAAACGCACCACCTCAAGGAACAGCTGACCGCTGTCGGCGGCGATCACCGCCGTCTGCGCCGCTGCCA GCCCGTGCTGCTGCATCAGCCGCAACGCCAGCGCCTCGGCGCGCAGCAGATCGCACCAGCGCCGGCTGTT TTCGTTGGCACGCGCCGGGGTAAATTTGACTAACACATGGGCCAGACTGCCGTCGGGCAGCTCGGACAGG CTGGCAAATTTGGGCTGTTCGCCCCCCGCAGAGGAGCCGGGTTGCTCGCCTGCCAGAACCTGGCTGGCCA ACTGTGGATAGCAGGTAGCCTTGTCAGACAGCAACACCGCCGCCGGACGCGGTTGTTGCAGCCACTGCTG ATAGCTGTGCTGACCAATCAGCCAACTGCCCGTGACGTCTGCCCCCCGGCTGCTCAGTGCCAGCAGACAA TGATCGTCGTTCCACTGGCGGATATCTTCCGGCAGCGCCAGTTGCTGCGCGCAGTCGCGCCCCCACGCCC GCCCGAGAAAGCCTTGCGGGCGCATATCGCTTAAATACCAGGGTAATCCGGGATAGTAGTGCCAGCTGGC GCTCGCGTCCTGCACCACGCAGCTTCCCGCCGGCCAGATGCTATAGAGTTGCCCGGCGGCATGGGCCTGA CCGGTTATATCTATTTGCCACAATGGGAACTGACTGACCTCGCGCAGCGGTCGCAGCAGGGCATAGCGCG TGGCGCGGGCGCGACCGTATTTCAGCACCTGCGGCTGGGACTTAATCAGGCGGGACAGCGTGGCCTGGCT GATGCCGAGCCGCGCCTGGAGGCTGGCGGCATCCAGCGGCCCCTGTAACAGCAGTGTCGTCAGTTCGCTC AT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 475138-476469	Sequence	EJP617_04310 yjjJ hypothetical protein (Protein is coded in complementary strand; from 476469 to 475138)