Sequence retrieval

Genome: Clostridium acetobutylicum EA 2018
Start position: 2158877
End position: 2159467
Length: 591

DNA sequence
>NC_017295, from 2158876 to 2159466 (591 bp); Clostridium acetobutylicum EA 2018 TTATCTTCCATGTAAATTTATTTTTGTTGGAAGATCATTAGGTTTACTTCCACAATTCTCAACAAATTCT AGGCTTTCATTCAGAGGATTATAGTTGTAATGATTAAGATTCATAATTATCATTCTTCTATTTGAAAGTT TAGTTCTTCTAGACATGCTTATAGCATTTTGTCTTTGAAGCATTAATAACACTTTCATTTCCTCTTCTTT ATTTACACTTCTTTTTCTCCTTATGGCTATTTTTTTTCTTTCTTTATTTATTTTTATGTTTTCCTCTTTT CTCCTCAACTTCTCATTTTTGTAATCTGAATCAGCTTTTATAGTCCTCGTTACTAAAGCATTACTTATAT TAAGTATATTGGCTATTTCTAAAACCTTCATATTTTTTTTATAAAACATTTCTCTTATTTTTTCTTGTCT TTCTTCTATTTTTGTTTTCTTGTTCTTTCTTCTAATTTTGCTTTCACTCTCATACCTTTCATTCTCTTTT AATATAGAGTATATATTTGTCCTACTTTTACCTAAAGTTTTTGCGATATTACTAACTGACATACCTTCAT TAAAGTACATAAAAAGCACTTCATTCTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 2158147-2158887	Sequence	CEA_G2066 - DNA-dependent RNA polymerase sigma subunit (Protein is coded in complementary strand; from 2158887 to 2158147)
ORF.. 2158877-2159467	Sequence	CEA_G2067 - hypothetical protein (Protein is coded in complementary strand; from 2159467 to 2158877)