Sequence retrieval

Genome: Thermus sp. CCB_US3_UF1
Start position: 2147176
End position: 2148993
Length: 1818

DNA sequence
>NC_017278, from 2147175 to 2148992 (1818 bp); Thermus sp. CCB_US3_UF1 ATGCCCTTATCCTACGCCCCTCCCCGTGGTAAAGTGGGGGGATTGGGTGCGCCCATGGAGATTAGAAACA TTGCCATCATAGCCCACGTGGACCACGGCAAGACCACCCTGGTGGACGCCATGCTTCGCCAGGCCAAGGC CCTGGCCAAGGCCGAGGGGGAGCGGATCCTGGACTCCCATGACCTGGAACGGGAGCGGGGCATCACCATC CTGGCCAAGAACACCGCGGTGGAGTGGCAGGGGGTCAAGGTGAACATCGTGGACACCCCGGGCCACGCGG ACTTCGGGGGGGAGGTGGAGCGGGCCCTTTCCCTGGTGGATGGGGTCCTGCTCCTGGTGGACGCCGCCGA GGGGCCCATGCCCCAGACCCGCTTCGTGCTGCGCAAGGCCCTGGAGGCCGGCCTCAAGCCCATCGTGGTC CTCAACAAGGTGGACAAGAAGGAGGCCCGCCCCGACGAGGTGCTGAACCTGACCTTTGACCTGATGGTGG AGCTGGGGGCCACGGAAGCCCAGCTGGACTTTCCCTTCCTCTACGCCGTGGGCCGCGAGGGCCGGGCCTG GCGGGAAACCCCCCGGGAGGACCTGGCGGAGCTTTTCGCCACCATCCTGGAGCACGTGCCCCCACCCCGT TGGCAGGAAGGCCCCTTCCAGCTCCTGGTGGCCAACCTGGACCACTCCCCCTACCTGGGGCGGGTGGCCA TCGGCAAGGTGACCCGGGGGCGGGTGCGCAAGGGGGAAACCCTGGCCATCCTGAAGGAAGGGCAGGAGCT CCTGGCCAAGGTGGCCGCGGTCTACACCCACCGGGGGCTGGAGCGGGTGGAGGTGGCGGAGAGCCTGCCT GGGGACCTCGTGGCCCTGGCGGGCCTCGAGGGGGCGGAGATCGGGGACACCCTGGCCTCCCCCGAGGCCC CCGAACCCCTCCCCCGCCTCCGGGTGGACGAGCCCACGGTGGCCCTCACCCTCACCGCCAACACCTCCCC CTTCGCCGGGCGGGAGGGGAAGCACGTGACGGGGCAGAAGCTGAAGGAGCGCCTGGGGCGGGAGCTGCGC ACCAACGTGGCCCTGCAGGTGGAGGAGGTGGCCCCGGAGGTCTTTGAGCTCCGGGGCCGGGGGGAGCTGC ACCTGGCCGTGCTCCTGGAGACCATGCGCCGTGAGGGGTACGAGTTCAGCGTGGGCCAGCCCCGGGTGCT CCTCAAGGACGGCCTCGAGCCCTACGAGCTCCTGGTGGTGGAGGTACCCCAGGAACGCTTTGGGGCGGTG ATGGAAGCCCTGGGGGCCAGGCGGGCGGAGATGGCCCACATGGAGGTGGGGGAACGGGTGCGGGCGGAGT TCGTGGTGCCGGCCCGGGCCCTTTTCGGCTTCCGCAGCCTTTTCCTCTCCCTTACCGGGGGGGAGGGGCT CATGAGCCACACCTTCCACGGCTACGGCCCCGAGGCCGGCCCCATTCCTAGCCGCACCACGGGCAGCGCC GTGGCCATGGAGGCCGGGGTGGCCACCGCCTACAGCCTGAACCGCCTGCAGGAGCGGGTGCAGTTTTTCA TTGAGCCGGGAACCGAGGTCTACGTGGGGATGATCGTGGGGGAGCACGTGCGGGAAAACGACCTGGACGT AAACGTCACCATCGGCAAGAAGCTCACCAACGTCCGGGCCGCAGGCTCGGACGAGAACATCCGCCTCATC CCCCCGCGGAAGCTCTCCCTGGAGGAGGCCTTGGGCTTCCTGGCCGAGGACGAGCTCCTGGAGGTAACCC CCAGGAGCCTGCGCCTGCGCAAAAAGGTCCTGGACCCCTCGCAGCGCAAGCGCCTGGTTGGCAAATGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 2146314-2147177	Sequence	TCCBUS3UF1_21640 - Major pilin protein fimA (Protein is coded in complementary strand; from 2147177 to 2146314)
ORF.. 2147176-2148993	Sequence	TCCBUS3UF1_21650 - GTP-binding protein TypA