DNA sequence >NC_017179, from 593384 to 593495 (112 bp); Clostridium difficile BI1 TAAAAGAGTAAAAGAGTAAAAGAGTAAAAGAGTAAAAGAGTAAAAGAGTAAAAGAGTAAAAGAGTAAAAG AGTAAAAGAGTAAAAGAGTAAAAGAGTAAAAGAGTAAAAGAG Translate to protein Restriction digest BLAST Design primers with primer3