Sequence retrieval

Genome: Shigella sonnei 53G
Start position: 830016
End position: 831158
Length: 1143

DNA sequence
>NC_016822, from 830015 to 831157 (1143 bp); Shigella sonnei 53G CTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAAC AGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACGAAAT AACGAGCGGGAATAATGTACGTCACCGCGCGGATCACCGCGGGCATACTGTCGATCTGAAAAATAAAGCC GGAAAGCATAATCGACGGCAGGAACGCGGCGTTCAGGGCGACCTGAGCGGCATTGAACTGGTTGCGGGTA ATCGTGGAAATCAGCAGCCCCATCCCCAGGGTACTGAGTAAAAACAGGCTGGAGATAAAAAACAGAATAA GCAGCGAACCGCGATACGGCACGCCGAGAATAAACACTGACACCAGCATACACAGCAGCATCGCCAGCAT CCCGAGAAAGTAATAAGGGATCAGCTTACACAGCAGCAGTTCCGTGCGGGTAATCTCCGTAGAGAGCAGA GCCTCCATGGTGCCGCGTTCCCATTCTCGCGCCACCACCAGCGAGGTGAGAATCGCGCCGATGACCGTCA TGATGATGGTCACTGCACCGGGGATAATGAAGTGCTGGCTAATCGCCGCCGGGTTAAACCAGTAGCGGGT TTGTACATCAATAAGCGGTTCAAAAGTCTGCCCGTTGTCCTCCGCTCGCTGCATTTGCCAGATCTGCCAG ATCTGCCAGATCCCTTCGACATACCCCTGTACAAAGTTAGCGGTATTCGGCTCACTGCCGTCGGTGATCA CCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACC GCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAG GGCGAACCGGTCATGGTGTGGGTGAAATCCAGCGCCGCTTCGCTACGCTGTTCCAGCAAGATCCCGACCC GCAGCTTGCTGGAGTCGAGGTTAATGCCATAACCAAAAATAAACAGCAGTAGCAGTGGGATCACTACCGC AATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGTCTCTTTGACGCACAGCGCCCGCACGCGACGC CAGGAAAGGATCGGGTTACTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 830016-831158	Sequence	SSON53_04240 - putative ABC transporter permease (Protein is coded in complementary strand; from 831158 to 830016)
ORF... 831151-832887	Sequence	SSON53_04245 - fused transporter subunits of ABC superfamily: ATP-binding proteins (Protein is coded in complementary strand; from 832887 to 831151)