Sequence retrieval

Genome: Shigella sonnei 53G
Start position: 259988
End position: 262777
Length: 2790

DNA sequence
>NC_016822, from 259987 to 262776 (2790 bp); Shigella sonnei 53G TCATAAAACCGTTTCATCCTTTGTGACGGACTCGTCGTTCTGAGCATCTTCCACGTCTGCCGTAAATGCG CCGTCCACTGCCGACAACCGAATCCGGGCAATCGCCGTGTTAGCCGCCATTTTCTGCAACAGCAGCAGCG AGAGCGGCGGTAGCATGTCGCCATCGATGACCGATTCCAGCATCCTCGCGCCGTTTTCCGCGCGGGTGAC GCGGCTCATGATTTCGTCCGTCACTTCCGGTTCAATGACCACTTCTACACCAAAGCGACTGCGCAGCACG TTATCCAGACGGGCCAGTTTTCCGGCGATAATGGTGGCGAGCGTCTCTTTCGACAGCGGCAGATACGGCA CCACTTCCATACGCGCCAGCAGGGCAGGTTTGAAGAAGTCGGCCAGCACCGGATACAGTACTTCCTGCAT GGTTTCCGGGTCATCGGCATGCTCGACTATTACCTGGTAGCCGAGGTTGGACGTCAGGAAGAAGACGATA TTTTTGCAGTCAATCAGGCGGCCTTCACCGTCTGCCATTTCGCCCTTATCGAACGCCTGGTAGAACAGGT TGAGCACATCCGGGTGCGCTTTTTCCACTTCATCGAGCAGTACTACCGAGTAGGGTTTCTGGCGAATCGC TTCGGTCAGTACGCCGCCTTCACCGTAGCCAACGTAGCCCGGAGGCGAACCAATCAGCCGCGAGACGGTG TGTTTCTCCTGGAATTCGGACATATTGATGGTGGTCAGGTACTGGCGACCGCCGTAGAGCAGCTCTGCCA GTTGCAGGACGGTTTCGGTTTTACCCACACCGCTGGGGCCAGCCAGCAGGAACGCACCGAGCGGGCGTCC CGGACGACGCAGGTCGGCGCGTGCGGTCAGTAGATGCTTATGCAGGCTGGCAATCGCCAGGTCCTGGCCT TTGATGGTGTCACCCAGCCATTTCGTCAGGTCGGTGATGACCGACATTTCATTCTGCGACAGGCGGTTGA GCGGCACGCCGGTCCATTCGGCAATCACCGCCGCAATCTGTTTTTTATCGACGTGCGGGGAGACCAGCAA CCGGTCGTTATGCAGGGCGTCCAGTTCGGCAGTGAGCTGGGCCAGCCGCTGTACCGGGGAAACTGTTTCA GCGGTCTCTTCCGGTTGTTCTCTGTCGGCCTCATCAGCCGGTTCGGCACCGGTATTATCCTGCTCTGACT CTGACTCTGACTCTGACTCTGACTCTGACTCTGGCTGCGTATCCTCCACGGTATCTGCGTCCGGCAACGG CGCCGCATCGTCCTCTGCCACGCCCAGTAACTGCTGGCGCAGCGCGATAATCTCCCGGACCAGCGTCTGC TGCTGGTGCCAGGCCGCTTCCAGTTCATCCAGCGCCGTCAGCGTTTCATCATACTGCACCAGCACCTCGG TCATCCGTGATGTGTCGGTACGCAGTCCGATGCGAAGCTCGCGCTCAAGCTGGCGAATTTCCGCCTCCTG CTGGTGGCTCAGAGTGGTCAGCGCCGAGATTTGCTTCGGCGGCGACGACAGGTTGATGGCCACACGGGCG CAGGCGGTATCCAGCACATCAATCGCTTTGTCCGGTAACTGACGCCCGGAGAGATAACGCTCGCTTAATG TCGCAGCGGCCTGCAAGGCGTCATCATCAATCAGCACGCCGTGAGACTGTTCATAGACCGCCGACAGACC GCGCAGAATAATGGTGGCTTCGGCAGCGTTGGGTTCGCTGACCTTCACCAACTGGAAGCGGCGCGACAGG GCGGCATCTTTTTCGAAGTATTTTTTGTACTCGCTCCAGGTGGTGGCGGCGATGGTTTTCAGCTCGCCGC GCGCCAGCGCCGGTTTGAGCAGGTTGGAGATATCCAGCCCGCCCTGCTGGTTGCCCGCGCCAATCAGGGT ATGTGCTTCGTCGATAAACAGAATGACCGGCACCGGGGAGGAAATGACCTCCGCCATCAGCCCTTTGAAA CGTTTTTCGAATTCACCCTTCACCGACGCCCCGGCCTGCAATGCGCCCAAGTCAAGGGTCATGATATCGG TGTTTTTCAGCTTGTCCGGCACCTGGCCTGCCACGATGCGCAGCGCCAGCCCTTCAATCAGTGCGCTTTT GCCCACGCCCGCTTCGCCCACCACCACCGGGTTGTTTTTACGGCGGCGGCAGAGAATGTCGATCATCAGG TCGATTTCGTGGTCGCGGCACAGTACCGGGTCAAGCCTGCCGTTACGGGCGTCTGCGGTCATGTTTTTGG CATAGCGGGCAAGCAGGGTGTCAGAGGCGTCCGTCATTGTGCCTGCGCCTTTACCGTCTGCATCCGGCAC GACTGATTCCGCCGACTCCTGTGTCCACTGCACAAAGTCCTGTTGCAGACGGTCACGGTTAATGCCGGTC AACAGCCGGGCGGCAGCAGGCGGTATATAACGCAGCGGCGAATGCAGCAGGGCCAGCAGCAACACGCCGC CGCGCAGTTCGCTGTGCTGCATCTCAGCCGACGCCAGCAGCCAGCCCTCTTTAAGCCACTCAACCAGCAT CGGGGAAAACGCCGGGTAGCTGTCCGCAGAACGGGCGGTTGTGTAGTTCTCTACCGTCAGCGCCTGGCGC AACTCTTCCATGCCAATATCGCCCTGGCGGGTAATAACCCGCAGGTCACTGCGTGGCATGGCGAGCATCT GAATGAGCACATGGCTGACGGTAATTTCCGCTGCCTGCTGACTCATACATTCAGAGGCGGCCATCTCCAG CGCCTGGCGGGAGAACAGGTTCAGCCGTTTTACCAGCGTGGGAAGATCAATCTGGATCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 259248-259991	Sequence	SSON53_01230 - type VI secretion-associated protein (Protein is coded in complementary strand; from 259991 to 259248)
ORF.. 259988-262777	Sequence	SSON53_01235 - ATP-dependent Clp proteinase ATP-binding chain (Protein is coded in complementary strand; from 262777 to 259988)