Sequence retrieval

Genome: Pyrobaculum sp. 1860
Start position: 722643
End position: 724556
Length: 1914

DNA sequence
>NC_016645, from 722643 to 724556 (1914 bp); Pyrobaculum sp. 1860 ATGAGACTCCTCTTTTTCCTCCTCGCCGCCTCCCTGGCCCTGGCGGCCACCATCGTCGATCCCAGGTGGG CCGTACCCGCGTACGTCACGCCGGGGGGCACGTTCAACATCACCCTCGACGCGCCGGTCGCAGTGAACAG CGTGGCGCTGGCGGCGCCTGGGCTAGACAAGCCCATAGCCCTCACATTCAAGACCTCCGGCAACGTAATC ACGGCGGCGGTGCCTGCTGACACGCCGCTCGGGCTGTACGACGTGGTGATAAACGGCGGGGAGCTCTACG AGCCGAAGGCTGTCTGGGTGGGCAACGTCACGGGGCCGCTTAGGATTCTCCAAATCACCGACGAGCACAT GGGAGTGGAGCTGGACATGGCCTCGGTTTACCGCCTCATACACGCCATGGTAGTGGCCAACGGCGGTCCC TTCGACGTGGTCTTCGCCACTGGCGACCAGGCGGACGTGGGGGGCCAGCCGTGGCACCACATGCTCTTCG CTAGGTACGCCTCCACCGCAACCAAGCCCATATTCGCCATTCCCGGCAACCACGACCACGCAGGCGACGA CCCCCTGGTAAACTACAGGAAGTTCCAAGGCCCGCCGGTGTGGTACAGAGTCGTCGGGCCCTACCTAATA ATCGGCCTCGACAGCGGCTTAGAGGGCTACCTCACCGACTCCGTCGTCAAGTTTTACGAAGACGTGTTGA AGAGGTACCCAGACAAGGTGAAGATAGTCCTCGTGCACCACCCGCCGTTTTACAAAGCCGACTCCTACAT AGTGGAGACATACAGAGGGCCGCAGGACGTCGACCGCCTAAACAGAGACCCCACCGGCAGAAGCCAGTAC TACATCGTCTACACCAGCTACCTCCAGAACAGACCCGCCTACGAGAAGTTCCTAGAGCTGACCATCAAGT ACAGAGTGGCGCTGGTGATGTCCGGCCACGTCCACAGCGGCAACTCCACCATTGTGATAAACGGCACGTA CTTCGTCACCACGAGGACCCTCGGCGGCTCCATAGACACCTCCCACGGCTACAGATCCTACGTCGTCTAC CCCGACGGCCGCGTGGAAATCGGCAGAGAGGTGTGGACCTACAAGAACTTCGCCGTCTTGACCTGGGGCT CCAAGGCGGCTCAGCTCTACATAGACAGCGACCTGGCGCCGGGCGAGATCACCATCGACCTCCCGAGCGA CTACTCGGGGCTGAAGGTGCTCAACGGCTCCGCCCAGCTGGTGAAGGTGCAGAGACACCCCCTGGGCAAG TACAGCAGATACACCATTAGGATCTCCGGCAGGCCGGTGTGGATCGCCATAGGCGACTACGCCCCGGCGC CCGCAGTCAAGGTTGAGAAGGTGCTCCCCAGGAGCCCAACCCCCGGCGACCTGGTGACCGTCACAATCTC AGCAGACGATCCCAACGTCGGCGTGCCCTTCCTACTGGTCAACGGCCAGAGAGTCCTCGGCTCCTACTTC GAGGAGAAGCCAGTCTACATCTACAAATTCACCTACCAGAAGCCCACGGCCCTCCAGATCCTAGCCCCCG GCGGCCAGCCCACCACCATACAGATAGGCCAAGCCACCCCGCCAGCCACAACCACCCCCACCGCCACACC GACCCAGACCCCCACGGCCACCACCGCCCCAGCAACGCAGACAGCCACAACAACCCAGCCCGCCCCCGCC ACCACCACCACGCCGATCGCCACGCCGCAACCAACCGCCGCGCCTCCGCCGTCCGCGGCGTCCCCGACTG TAACCGCGCCGCCAGCCACACCTGCGCCCGCCGCGCCGCCGGCGCCTCAGCCCGGCTTCCCCATTGAGGT AGCCGCGCTGGTAATAATCGCGGCGGTTGGGGCGGCGGTGCTTGCTGTGCTTGCTAGGAGGCCCAAGCAG GGCGAGACTCGGGTAAGGTCCTAG

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 721321-722643	Sequence	P186_0841 - hypothetical protein
ORF.. 722643-724556	Sequence	P186_0842 - metallophosphoesterase
ORF... 724553-724756	Sequence	P186_0843 - hypothetical protein (Protein is coded in complementary strand; from 724756 to 724553)