Sequence retrieval

Genome: Pyrobaculum sp. 1860
Start position: 279232
End position: 285132
Length: 5901

DNA sequence
>NC_016645, from 279232 to 285132 (5901 bp); Pyrobaculum sp. 1860 GTGTCTGACTGGGTTGCGGCTCTCCACCTGGCGGCTGCCTTGTCTGCTCTGTATCTACTGTTGTGGAGGG GGGATGAGGAGTGGTATTTTCCCATATTTGCAGTTGTCACTGTGGTGTGGGCTGTGCCGCTGGTGACGGG CTTTGTGCTTCAGTTCTCCGCCCCCTACGTCCCGAACCCCCTCTACATCTACGGCTTCTACGAGGCGCCT GGGGGGTGGGCGGAGAGAATTAGAGCCACCGCCGAGGCGGCAAGGCTGGCGGCTGAGGCGGCGGCCTCCG CGTTGGAGGGGGCGGCGGTGGCCTCTTCTGTACTACAGACTTTGATGTTCCTCGGCGCGGTGGCGGCGGC TCCGCTCACCGGCGGGGGGTCTCTGGCGGCGGCTGTCTTCGCGCTCGCCGCCTGGCGCTACATGGACCCG GTGTTTCAGTTGGCCAACGCGGCGTATCAAGCGGCCGTTGGGGTGTATATGGTGTTCCACTTGCTAGAGA TACTCGCCTCCTGGGCTGAGAAGTGGTTTGGCCTCTTAATGGCGGCTGGGCTATTCCTCCTGCTGTTCCC CCGCGTGAGGACTTGGGGCGCCTTTCTCACGGCGCTGGCGCTGGCGTTATACACAGCCGCCGCGGTGGGC GCCTACCTCTCGCCGTTAGGGCTGGCGGTGGCCCAGTGGGCGGAGGAGACGGCAGAATGGGCTAAGTCGG CCCCCAGCTCCACGGGCTCTCTCTCGTATCTCGCAACGCGGGGGGAGGGGGTCGCCCTCCTCCGCTACCA CAACTACTTCTCACGCCGCTGGCTTGTGGAGGAGCTTAACAAAACCTGGACCTCTCTAAACCTCTCTAGG GGGGTTCCCAGATTTGACCTCCTCAGCGGCGTGAAGATACTGGCGGTTAACGGTAGTGACTGGGCGGCGG CGGGGCCGGGGGCGCCTGCCGTGCTCTACGTGGGGAGGAAGAACTTCACAGGGCTGGGGGCTGGCTCCCC ATACGCCGTCTACGTCTGGCTGGACTACCCAGCCTCTATTACAAACGCCTCCCTCTGCCTCAACTACCCG GAGACCCCCCTGCCCAACGGCACGATGCCCTCCGGAGAGAGCGGCGAGCGGTGGCTTAGGAGGGCTAGGG CTGAGGAGTGCCGACTGCTGTCGGCGCTTTACCCCCTCTACGTTGTAGACACCTCTAAGCCCAGCCATTG GCGGTTGCTACACGCCGGGGTCAACGCCACGGTGACATACGGCGGTGAGTTAGAAAGGGGGGAGAGGAGA GGCGCCGTCGGGGTCTGGGGCTGGGTTGTTAAGCCGGATGTCGAGGCGTGCGGCGTCGGCAACGCCGCTG GGGACTGCCGCGCGCTAGACGTCGCCGAGAGGCCAGGCCAGTTGAAATACGACGTAGGCTGGGACAGGCT GGAGTGGAGGAGGCCAGTTTTAAACACGGCGTGGTCAAACTTCTCTATTCTTCTGCCCAACGCCCCCAGA CCTGTAAAAGACGGCTCTATAGACTTCTACAAGTGGAGGCGGACTTGTGAGTGGTGTTGCCGCTACGTCA ACGGCACGTGTGTAGAGCGGTGTTCCTCAACTAGAGAGGAGTGGCAGGAGCCGCGGTACCGCAAGAAGCT GGAAACCCGCCAGCTACAGATAGCTACTATACACCTAGGTAAGCCCTGGGAGCCCTTGGAGGTGCGGAAC GGCGCAGAGATCCCGAAGGTGGACGCGGCTATGCCCTACACCTATAGAGACTGGGAGGTTTGGCACGAGG TGAAATACGGCGATTGGGAGGGAGACGGGGAGCCTCCCCCCGCCGCCTACTGCTACGTACACGACAACCG CACCTATATGAGAATTAGGTTTTGGGACGTCCAACGCGCCGCTAGATACGTCTACGGTTTTGTCTGGATG CGGGGGGTCGCCGTGGAGGTGGACGACCGCCGCTCCGCGCTTCCAGATGAATACGGAGACGCGCCGGTTG CCGACGGCGACGGGGTGGCGGTGGTGAATCTCCTCCGCGGCGCGGAGTACGACCAAGAGTGCCACGTGGC TTACTACGGCGCAGATGAGCCAAATTGGCGCCACGTGCCCATGGCGGCTGGAAACATGTCTAAAACCTCC CGGGACTACTTCCTATCTGTCCTCGCAGGTGGGAACTACAGCCGTAGATACGTAGAGGCCCTCTCCCGGC TGATTCCGCCGAATACCTCAAGCCCGGTGGCTGAGGCGGCGGGGGGCGAGCTGGGGTACTTCGCGTCTAT TGCCCCGAGGCTCTTCTTCCCAATGCCTGACCTAGAGCCGAGGAGTTATAAAGCCGGGCAGTTTAAAATA CTCTGCGCCCGCTTCGCCTGGGATAACAACGGCGAGGGCGGCAAAGCCGCCTGGGGGAGGCTCCTCCTGC ACCCGGGGGAGGAGGTGTGGCTCTTCGGCTACTCCCTGGGGGATTCCAGAGCCACGAGGGAGTCTATCTC AGCCCTGGCGGAGAGGTGGAGGTGGATGCTGGCGCACCCGCCCCCTCCCCCGCCCGCCTCGTTGGCGCCT CGCTGGCCCATTCCCTGGAATAACTGGACCTCCCTCCCATACAAGCCGGCGGACGCGCCCCCAATGCCGC CGCCCGACCCCAGGTGGGGCGTGTCTATGTGGGACGTGGGGCTTGTTCTAAACCCCATCGAGCTCTTCGG CACGTTGCTGGGTAGGCTCTGGTGGAGTATCTACGTGGCGTTGCTGACGCCGGTTTTTGTCTTCGAGGCG TTGGCGGCGGTGTTCGGCTTCCCCTCCGCCTCTCGCTATCTGCTTTACATAGTTATAAACGTAATTCAAG ACCTCACCTACTGGCTGGGCATTAGGCTGTTTCTCAAGGCGAGGCTGGCGTATAGGCTGGGCAGGGCCGC CGGCTCCCCAGTCAAGAGGGCCTCCGCCAGGCTGGTTAGGAGGCTCGCCCACAGGGGGTGGGCTAAGTAC AGAGAGAGGCCGGGGGAGCGGGCTGTTAGACGCAGATTCGAGGCCTACATAGAGGAGAGGATACACGAGA GGATAGGGAAGGTGGTGGAGAAGGCGTTTACGAGGGCCGAGAGGGCTGTGGAGAGGGGGGTCGCGGTTGC GGCTGAGGCGGGGAGGTGGGCGTATGAGCTGTACAAGAGGGCGCAGCCCTACACCGAGATGGACGCGTTG CAGTGGCTGAGACAGCTCTGGCCGCAGTTCGACGCGAGGTTTGAGCAGTGGATGCGCGAGGTAGCCCAGA GACGTCCCGCGGCCTACTCCCTATTTCTATCGCAGATAGACGACAAGCCCCGCTGGATTGCCCTAATCAA CCCTGCTTATCTCCACAGACTGCTGGCTGAGGGGAGGATTAGCCAAGAGCAGTACCAACAGCTCATGGAG CTTAGGCGGTACGTAGCCGCGGCTAGGGCGGCTCAGTGGGCGAAGTACGTAAGGGAGAGAGTCGACGAGG TGGCCGCCCTCCGCGCCGCCGTGGAGCTACACGAAAGAGCTGTGAGGCAGTCCACCGGGGCCTTCTACGC CCTTCTATACGACTTGGCGGTGCTGGAGGCCTCAGATCTAAAGACGGCTGTGGAGCGGGTGGAGGCTAGG CTGAGGGCTGTCTACCAGCCTGTTGCCAGAATGCACCCCAGGGCCTTCGACAGACTGCACCAGGTGTTTC TAGGCGCCGGGGCGCCTAGGGAGATCAGCGGGCCGAGGGAGCTGGCCCAGTGGCTTGTGGAGAATAGGGA GAGGGTTCTGGAGGCTGGGGGGAGGTTGCTGGAGGCGTGGAGAGATGTGTGGATTGCCCACTGGAGCTCG GCCAAGACGCCCTACTCAGCCCACAGATATGCAGAGGCGGAGGCGTTGCGCCGAATGCATCTAGAACTGC CGACGCTGTACGTAGTGGGGCTGGCCAGGGGGCTTGTGCTGGGCGCCCGCTCTCTGGAGGAGGGTAAGAG GGTTTTGGAGACAAAGACGGCGCGGGTTAAGTCTGTGGAGGTGGTGGCTGTGAGGCCTGGCGAGGCCGTC GGGCCGTCTTTCTTCGGCGAGGGCGCGGCGGAGCTGGGCCGCGTCAAGGCTGGCGAGGTGGAGCTGGTGA GAGTGGAGAGAGTTAGAGAACTCCACGAGGCGGAGGGGAGGGCCCTGCGGCTTTTGGAAAAAGCTGTGAG GGCTGTGGAGGAGGCGCAGAGGGCGGGGGGAGGCGGAGGGGCCGTGGAGAAGGCGCTTGAGAGGCTGGAG GAGGTGAGGAGGGAGGTTCTTGAAGAGAGCTGGCGCTGGTTTAGAGAATTTGGCGAGCGGTACCTCTGGG TTAAGTACGTCGGCGAGAGGATATACGCCGTCGAGAGGCCCGGCGCGGCTGAGATGCGGAGAGCCTTCGA CGAGGCGTTGAGAGTGTTTGTAGAGAGGGGGAGGGAGGAGGCAATTCGAATTCTACACGAGAGAGCCGGG GAGCTACGCGTGTTTTATCAACAACGCGGCGGCGTCGACGAGGTTAAGACCGTGGAGAGGCTGGAGAGGG AGTACGCCAAGCTGTTAGAGAAGTTGCCTACAGACGTCGCCAAGAAGATGGCCTCTGTAGACGTGGTGAA GCCGGGGGAGAGGGCGGCCGCGCTTAGAGAGCTGTTGGAGAAAACCGCCGACGTGGAGACCGCCAAGAGG GCTCTTGAGCTGTATGCAAGAGGCGAGGGGCATCTGGCAGAGGCGGCCTGGGCGGCGAGGCGCGTCTTAG TAGAGGTGGAGAACACGCTAGCCGAGCTGGAGAGGGTGGCGCGGGAACACGCGGCGTTGAGAAGGCAGGC GGAGGAGGAGCTGGCTAGGTTTGAACAACACAAGATGAGCCTGAGACGGGCCTTGGAGGAGTATCTAAAG TGGAGGAGGGAGGTGGCGGAGGCCGAGGCTGAGTACAAGAGGCTGAAGGCGGAGTTCGACAGGATCAGCG AAGAGGTGGAGAAGTGGAGGAGGGAGCTGTCGAGGCGCGTTGAGGAGTTGGAAAGGCTGGCGGAGAGGGC TGTGGAGGCCGGGGGGTTTGCCGAGGCGGAGAGGCTTAGGCAAGAGGCCGAGTATCTAAAAGCCACCGCC TCCCTCAACCCGGAGAGGCTTGTACACGCCGAGAGGGCGTTGAGAGAGGCGGAGGCTAGGCTGGCGAGGG CGGAGGGGGAGGCGCGTAGAGCCGAGGCGTATTTAAAAGCGGTGGGGGCCGAGGCGGCCCTCTCCGCGGC TAGGCTGAGGGAGTTGGTTAGACAGATGCAGGTTTTAGAAAAGGCGGAGGAGGCGCCCATGGCCCTTTAC TACATCCGCGACCTCCAAGGCGCCGGGGTGGAGGAGCTGGGTAGGTTGTTGTACAGAGCTGTGTATGCGG AGGAGAGCGGTGGGTATCTCCCAGATCCTCTGCTGTATCTTGTCGCCGTTGCGTTAAGAGAGCGGTGGGA GGCGGCCCGCGCCAGCGGCGACGTGGGGAAGATAAAAGCTGAAGCTGAGGCGCTGTGGGAGTTGGTAAGG GAGAGGAGGGGGGAGTTCCTCTCCGTGTTGAGAGAGGTGTATAGAGGCAGGGAGCTGGAGGTGAAGCTGG GCGTAGTGCGGGTGGATGAAGACGCCCTAGGCGCCTTGGCCGAGGCTTTTAAGACCGCGGAGAGGGTAGT CCAGCTGGAGAGAGACGTGGAGAGGGCGAGGAGGGAGGCCCGCGCCGCGGAGAGGCGGGCGAGGGAGGTG GAGAGGCTTGTGAAAAAAGCTGTAAAACAGCTGAGGCCGCCCGACGTTGAGAAAACTGCCAAGCTGGCGG AGGAACACGCCTCTCTCGTAAAGACGCTCTCGCAGACGGTGGCCGGGGTAGAGGAGGCGATGGCGAGGGG GGAGTGGGGGCTGGCGGCGAGGAGCTGGCGGCTGAGGCCGTCTACAGGGCGCTCGGCAGGTGGGCCGAGG TGTCGAGAGATGTCTATATGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 279232-285132	Sequence	P186_0328 - hypothetical protein
ORF... 285129-289898	Sequence	P186_0329 - PaRep2b-like protein