Sequence retrieval

Genome: Pyrobaculum sp. 1860
Start position: 208598
End position: 209848
Length: 1251

DNA sequence
>NC_016645, from 208598 to 209848 (1251 bp); Pyrobaculum sp. 1860 ATGGCGGAAAACGGTAAATTCCTTAGCTACGTGCTTCAAAACTTGAAGAGAGGGGTTGAGCTCGGCGGCT TTCCAGAGGACTTCTATAAAATAATTTCTAAGCCGAAGAGAATACTCCAAGTCTCTATCCCTGTGAAAAT GGATAACGGCAGGTACGAGGTGTTTGAGGGTTATCGTGTTCAGCATTGTGACGTTCTTGGTCCTTTTAAG GGTGGTGTTCGTTTTCATCCTGAGGTTACTCTTGCCGACGACGTGGCTTTGGCTGTTTTGATGACGCTTA AAAACAGCCTCGCGGGTCTTCCCTACGGAGGGGCTAAGGGGGCTGTTAGAGTTGACCCCAAGAAGCTCTC CCAGCGGGAGCTTGAAGAACTGTCGAGAGGCTACGCCAGAGCCATAGCCCCGCTGATAGGAGACGTGGTT GACATCCCAGCCCCAGACGTGGGGACAAACGCCCAGATAATGGCATGGATGGTAGACGAATACTCCAAAC TAAAGGGCTACAACGTGCCCGGCGTATTCACCTCCAAACCCCCAGAGCTCTGGGGAAACCCAGTAAGAGA ATACGCCACAGGCTTCGGAGTGGCGGCGGCGGCGAAGGCGGCGGCCGACAAGCTGTGGGGTGGGATCGAG GGTAGAACTGTCGCCATCCACGGCGCGGGAAACACGGGCGCATGGGCCGCCTACTGGCTGGAGAAAATGG GGGCGAAGATCGTAGCGGTGTCGAATACCAAGGGGTCTGTCTTCAATAAGGTGGGTATACCTGCCGACGA AATTTTGAAAGTCTATACGCGCGAGAAATCGATACTGGAGCTTGAGGGGGAGAAAAGCGAGAGTACAGAT CTCCCGCTCTATGTAGAGGCCGACGTGCTTATTCCTGCTGCTATTGAGAATGTTATTCGTGGGGATAATG TGGGTGGTGTTAGAGCTAGGGTTGTGGTGGAGGGGGCTAATGGCCCTACTACGCCGGAGGCTGAGCGGGT TTTGTACCAGCGGGGTGTCTTGGTGGTGCCAGACATCCTTGCAAACGCCGGGGGGGTGGTGATGTCCTAC TTGGAGTGGGTGGAGAATCTCCAGTGGTATATATGGGACGAGGAAGAGACTAGGCGCAGGCTGGAGTCTA TAATGACCACCAACTTCAACCGGGTATACCAGAGGTGGGAAAGGGAAAAGCAGTGGACTATGAGAGACGT AGCGATAGTCACAGCCCTAGAGAGAATATACAAAACAATGAAAACAAGAGGATGGATCTAG

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 208598-209848	Sequence	P186_0238 - Glu/Leu/Phe/Val dehydrogenase
ORF... 209845-210051	Sequence	P186_0239 - hypothetical protein (Protein is coded in complementary strand; from 210051 to 209845)