Sequence retrieval

Genome: Pyrobaculum sp. 1860
Start position: 2017170
End position: 2017454
Length: 285

DNA sequence
>NC_016645, from 2017170 to 2017454 (285 bp); Pyrobaculum sp. 1860 CTACTTCATCCGCCTCTCCAACTCGCCGCAGACATCCGCGAGAGAAAGGCCGAGTGAGTTCAGCAACACC AAGAGGTGGTAGAGGAGGTCAGCCGCCTCGGCCACCACCCTCTCCCTCCCCTCTGACAAGGCCGCCACGG CCACCTCCACCGCCTCCTCACCGACCTTCCGGGCTACCGCGGGAATGCCCGAGGAGTATAGGCGGTATGT GTAGCTCTGGGGGTTGCCCTCGGCAATTCTCCGGCGGATGACCCCCTCAAGCTTCTCCAGCACCTGGCAC GTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 2017170-2017454	Sequence	P186_2420 - phosphoribosyl-ATP pyrophosphohydrolase (Protein is coded in complementary strand; from 2017454 to 2017170)
ORF... 2017451-2018590	Sequence	P186_2421 - histidinol dehydrogenase (Protein is coded in complementary strand; from 2018590 to 2017451)