Sequence retrieval

Genome: Pyrobaculum sp. 1860
Start position: 1992866
End position: 1994065
Length: 1200

DNA sequence
>NC_016645, from 1992866 to 1994065 (1200 bp); Pyrobaculum sp. 1860 CTAGTCGTGTTGCGACTCCTCAACCTTCACCCCCTTTACGACAGCCTCCACGTACCTCCTCCTATTCTTC TCATACTGATCGGCGGGGTACGCCTTAATCTTGGATAATATCTTCGGTAGCGCGGTGTACTCCATCTCCT CCGGCGGCAGTCTGTGGGGCTGGAACGGCCCCATCCTCCTGATGTAGTCGGCCACCTGCGCCGCGACTTG CCTCACGTAGTCGAAGGCTGGGTCGTCAAAGAGGTCCACACCCCCCTCCAGATACCCCTCGTGGAGCTGG AACCCGAGGGCGCCTACCTTCGGCGGCCCGTCGAACCGCGTCATCTTGGCCCCGATGGCGATCCTCCGCT CGGGATCGACTGAGATGAAGCGCGCCGGCATCAACGGCCCGGCGTGGCTACCGCGCATCCAGCCGTGCAC GAGGTGTGGGTGGGCGAACGCCTCGAGCACCTCCCCCACAGCGGGCAGGCCCGACTGCGCCCTGACCACC ATCACCGGGTCGTCTTTGCCGACGTAGCGGCCAGCTATGAGGGAGAGCCTCTCGACGCTGTTGGCGGCGG CGGGGGCGCCGTCGGCTTTTCTAAAGACTTTCCTAATTATATACCTCCCGGTGGTTCCTATAAGGCCGAG GAGGGAGTAGGCCTCCTCCGGCGTCTTGAGCAGATATACCTTGTGCTCAACTACGTCTAGCACCTCGAAT ATGAAGCCTTCGTGCATCGAGGGGTCTATGACGAGGCCGGCTGTGTTGAAGGGGTCTGCGAACATTTTGT AGAGGGGGAGGTTGAAGGCGCCTGGCTCGGTCTTGTCGGCGGCGAATACGATCAATGGCTCGCTGGGCCT CTCCTCGAACTCCATCTCCGCAACCTGGGGGCCCATGCCCCTGACGTTGCCGGAGAAGGCGTCTTTCAAA AGGTCCTGGCCCGCGCCGTACAGCTTGTACCTCTTGGCTATCTGCTCGGTGACCTCCTTGAAGGTCTCCC ATGCAAGGCCGTGGATCTCGGGGTTGTCTTCGCCTTTTGTGTGAGTCATTAGTAGAGATATGTCGTCTCC CACGTTGTAGACGAAGTAGTCGATTATGAGACCCCTCTTCTGGGCCTCTTTCAATTTCGCCGCGGCGTAC TCAAGCATTTTTGGGTGGACATGGGCGTGACCTGGGAAGCCGCCTACGTCGGCTTTAATTATGGAAACCG TTACCTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1992866-1994065	Sequence	P186_2393 - fructose-1,6-bisphosphatase (Protein is coded in complementary strand; from 1994065 to 1992866)