Sequence retrieval

Genome: Pyrobaculum sp. 1860
Start position: 197342
End position: 198589
Length: 1248

DNA sequence
>NC_016645, from 197342 to 198589 (1248 bp); Pyrobaculum sp. 1860 ATGCCAACCTGGACCGAGTACATATTCTACAAAAAAATTGGCCGCGTCCCCTCGCCAGGTGACGTCGTCG AGGTGGTGCCGGACCTCGTGGGGTTCCACGACTTGACTGGCTACCACGTTCTCGAAGTCCTGGAACACAT GGGGAAGGTGGAGGTCTTCGATAAGGGCCGCGTAGTGGTGGCCTTTGACCACCTCTCGCCGGCGCCTAAC CAGAGGGCGGCGGAGATCATGGTCTACATAAGAAAGCACGTCAAGTCGCTGGGCCTACCCAACTTCTACG ACGTGGGGGGCGGCATACTCCACCAGATAATACTGGAGAAGTACGCGCTTCCGGAGCAGGTGATCTTCGC CGCCGACAGCCACACCAACACCGCCGGCGCCGTGGGCGCCTTCGCCCACGGCATGGGGGCCACGGACATA GCCGCCGCGCTGAAGCTCGGCAAGACTTGGCTCGTGGTCCCCACGCCTTTTAGAGTAGACGTGGCTGGGG AGTTCCCCCCGGGCGTGGTGGGGAAGGACGTGGCTCTGCACCTACTGGCCCAGTTCGGGGCGGAGGGCTT CAACGGGTACTCGGTAGAGGTGTTTGTAGAGAAGCCAAAGGCCTTCCCAATGGACGACAGGGCGACCGTG GCCAACATGTCCACTGAGATGGGCGCCGACGCCTTGATGTTCATCCCAGACTCAGTCACCGCCAGCTATC TGAAGACGGAGAGAGGAGTGGACTACACCCCGCCCAGCATAGAGCCCGGCAAATACGCCGACAAGTACAC GGTGGAGCTCGGGAGGCTTGAGCCGCTGGTGGCCGCGCCATACAGCGTAGACAACGTAAAATCCGTGAGG GAGGTGGAGGGCGTGGAGGTGGACCAGGTGTTTATAGGTAGTTGCACCAACGGGCGGCTCAGCGACGTGG AGATTGCAGCCAAGATTCTGAAGGGCCGTCGGGTGAAGACCAGATGCATAGCCATACCCGCCAGCTACGA GATATTCAGGAGGGCTATGAAGCTCGGCTACATAGACGTCTTGACAGAGGCAGGTTGCGTAGTAACCTAC GGCACTTGCGGCCCCTGCCTCGGCGGACACTTCGGCGTCGCCGGGCCTGGCGAGGTAGTGGTGACGACGA GTAATAGAAACTTCAAGGGGAGGGTGGGCCACCCCGACTCTAAGGTATATCTAGCAAACCCCGCCGTCGC CGCCGCCGCGGCCGCCGAGGGGAAGATAGTAGATCCAAGACCGTACCTCAGAAGTTGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 197342-198589	Sequence	P186_0223 - 3-isopropylmalate dehydratase large subunit