Sequence retrieval

Genome: Pyrobaculum sp. 1860
Start position: 1879021
End position: 1881705
Length: 2685

DNA sequence
>NC_016645, from 1879021 to 1881705 (2685 bp); Pyrobaculum sp. 1860 ATGTTGGACTTTCGGCTAGACTCCTGCCAGGGCGCCATTGTTGAGGAATACCTGTCCGAGGGAGCGAGGA TTCAGATTTACCAATCGGAAGACGGCTACTGCTACAACGTGGCCTATGCATTCCCCTACTCAGAGAGGGT AGGTGAATATGCGTATAAGGTAGTGGAATACCTAACTGCCAACCAGATAATTAGACCCAACATCTCAAGG GAGGAGCTTGGCAAACTGATAGAGATGGCAATGACGGACATCGGGGTACCTAAGCAACTGCGCGCCGCGG TTAGGTACTACGTCCAGCTCGAGGCGGCGGACTACTCCTACCTAACGCCTATACTATATGACATCAGACT TGAAAATGTCAACATAAATGGAACTGACAACCCAATCTTTGTAGATCACCGCGATTATGGATACAACGTA AAGACAAATGTTATACCAACTAATAGGGAGTCATTAATAAAAATCGTGGGGAGAGTCTACGCGGAGACCG GAAGACCACTAAACGAGCAGTACCCAATTCAAGACACTTATATCAGGCTGAGGAACGGGGCACTACTGCG CTTCGCAACTGCGATGTCCACACGCGTATCGAGGAACCCGCCGTACGTATCCGTGCGTATCCAGCCGCCC TTCCCAATATCCCCCACTGAGCTCATTAAGAAGCGTACCATCTCCGCGCTTGCGATGGCCTACCTCTGGT ACCTCTTCGAACACCATAAATCTGTTATGGTTATCGGAGGCACGGGCACCGGCAAGACTACATTACTAAA CGCACTGCTTGTCCTCCTGCCTCATAAACGCCTCGCCATCGCCGAGGAGACTCCGGAGATAAGAGTCCCC CCGAGCTACCAGAACGTGGTAATGCTCTTCACATCGCCCATGTACGACTACATGAAGCACCTGCCTGGGT CTGAGTCCGCCATATATCTAATAGACTTGGTTAAGTACCTCCTACGCGCCAGGCCCGACATAATTATAAT CGGCGAGAGCCGCGGTCGCGAGATACACGAGCTGATACAAGGCGTGTTGACAGGCCACGGAGGCGCCACC ACGTTCCACGCGGAAGACATCATGGAGGTCTTCATGCGTCTCACTGGAGAGGCCATGGGCGTCTCGGCGG AGCACCTCTCCGCTTTCCACGTGTTGGCCACTATCAGAAGATTCGAATTCGGTAGGAGGGTCACGTCTCT GACGGAGGTGGTGTGGCTGAGGGCGTATCCGTACGCCGCCCCAGGCAAGATAAAGATTAAAGAGGAGGAG TTTGGCTTAATAAACGTGGGTTGGTACGACCAGCGTACAGACACCGTGGAAGTGGATCTAAGGAGATCCT ACTGGCTACAGAAAGTAGGGGGGTACGACGAGATCCTCGAAAGAGCTAAGTTCTTGGCAGGCGGCAGGGC GGTAGACGTTAAGAGAGTTTTGGGCTGGGATGACGTGGTCATGACCGACTCGGGCGCCTACCAAATAATG AGGTACGGGAGTATCGAGGTGGATCCCGACGAGATTTTGCTTTACCAGGCCAGCATCGGGAGCGATATCG GCGTAATACTAGACCTACCCTTTGACTACGAGGAGCCGTACGAAAGCGCCTTGCTAAAGGTAGAGGAGAC TATCAGGAGAGCAAAAAGAGCCGCGGCTATGCTGGAGAGGCTAGATATGTTGGTTGTGGGCCCTATACAA GGCGGTCTGCATCTAGATCTCCTGACTAGATCCGCGCGTGAGATCTCTAAGCTCGGCTTCCACATCTTCG CTATTGGTAGCCCCACGACGTTGCTTGAGGAGTATAAATTTGACTTGTTGCTTGAAGCCGTACTGCACGT CAAGGCAAATATCGTGAGAGAGGCGCCTCTACACCTCTTTGGCGCTGGTCACCCACTAATCCTCCCGTTT GCGGTTGCTCTAGGTGTCGACCTCTTTGACAGCGCTTCCTACGTGTTATACGCAAGAGACGGCAGGATAA TGCTTAGAGATAGAACCCTCCGCCTTGACGATGTGAAGACAGATTACCTCCCCTGTAGCACGAAGCTGTG CTACACGCCAGTAAAAGAAATACGGGAGATGACCAGAGAAGAGCGCACTCAACTAATCGCCGAGCACAAC CTAGCAGTTCTGAGAGAGGAGCTCCTCGAGGTAAAACAGAGAATACACGAGGGGACGCTGTGGGAGTACC TCGAGATAAAAGCCAGGGCACACCCATCGCTGTACAGGTTTCTAAGAGGTCTTGCAAAGTTTAGGAAGCT TATAGAGGAGTACGACCCCGAGACTCACCCAAAGCCGCACGGACTCTTTTTCTACCAAGACACCGCCGAC TCCCGGCCAGAGCCACTTAGACACTGGACTAGGATGGCGAATCTGTACACGCCCTCTAGAACCGCCGTGG TGATTAGGGTAGACGAAAAACCCTACAGCAGAACTTGGGAATACCGCTACGTCAAGAAGTTAACCGGGGG AAGGGCCCACATCCTCTTCTACGACCCCGTGTTTGGCATTGTGCCGGAGGAGGTGGCCGAGGTGTACCCC CTCTCCCAGAACGAGGCCGAGGGCGAGGGCGAGGGGGCACGTGCCCATGCATACGAGTGGCTAAGTCAAT ACGACGTCGTCTTGACGTACAACGTCGATTTGCCTATTATTGGCAAAAGAGTCGTGAAGCTAAGGACTCT TGACGAGGTGTCTCTATATATATAG

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1879021-1881705	Sequence	P186_2260 - type II secretion system protein E
ORF... 1881694-1882410	Sequence	P186_2261 - hypothetical protein (Protein is coded in complementary strand; from 1882410 to 1881694)