Sequence retrieval

Genome: Pyrobaculum sp. 1860
Start position: 1568240
End position: 1569487
Length: 1248

DNA sequence
>NC_016645, from 1568240 to 1569487 (1248 bp); Pyrobaculum sp. 1860 TTATACACGGAGCCTGGGAGCCAAGATTTTTATCTGAGGGACGTGTAGCAGACGCTGGAGTGTCCCGTCT CTCCAGCTACCCACCAGCGCTATGAATAGAGCCACGGCGTAAGCGGCGGCGGCGCCGGCGGCGACGTTTC CGCCGAAGAAGTCGTAGAAGTGAAGCACCGGGGATAGGTAGATGGAGGTGGTGAAGAAGAAGGGCCATAC GTACGACAGTATCGTCATGCCGGCCACCATGCCGAACCAGGCAATTAGATGCGGTATGCTACCCTCCCCG CTTCTGTAGGCGCTACTGAAGCTACAGCCCGCCACGAGGACCATCCCCACTCCGAAGAGCACCCCGCCGA GGAAGGTGTGGACGCCGACTGGGAATAGGAACTCCTTGTACTGCCAGCCAAAGCCGTATTTAAGCACCAG GTTGCCGGTGATCATAATGGCGAGGGCGATGGCTATGGCCCTCGCGTTGCCACCGTAGGGGGCCAGCCTT GACCAGAAGCCGTCCCTCAGCATCGAGGTAAAGCATATATTGCCCTTGGAGCCGAAGAATCCGTAGGCGA TGCCTAGGTAGAGAAAGGCGATCGCGGCCCAGATACCCAGCCCCAGCCAGTAGAGAGTGGTCAAGGCCGC CGCGGCGAGGGCCACCGCCAGCACCTTGCGGTTGTCTACGCTGGAGCCCAGCTCAATCCTTATGGGCTTG GATCTATGCATCTTCTCCATTAGGAAGTCCTCGATGAGGCGCGTCGAGATGAAGGTGCCCACGGCCATCC CCAGGAAGAAGACGAAGCCTGAGAACACCAGCGACTGTATCGTCGCGACGAAGTTACCCACGTTGCACCC AAGAGCCAGCCTCGCGCCGAAGCCCAGCATGAGGCCGCCGACGATGGAGAGCAGAACCACCATCCTGTTG GCCGGCCACCGGAGGCGCCAGTTCCCCTCGAGGGCCTTAGCGATCATAGCCCCCAGCATAAAGCCGAGGA CTATCATAAACTCCTTATTTTCCGGCTCCCACGGTGCCGCCGCCGGGGCTACGAACATGGGGGAGCTCGG GTTGAAGTAGTCCCAGGCCTTTGTGTTAAGGAGGCCGCCGGTAAGGACGTAGAACAGCCAACCCCCGAAC TGGTTCTCCGGCCCCGTGATGTACCACGGGCTCTTCACAATAATTATCTGCAACACGGCGGTTAGGCCTA TCAAAGCCCCCGCCACCTCGGGCCGCCAAGGCTCTTTTAAAAACTTGGGGAGCGGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1567069-1568247	Sequence	P186_1863 - hypothetical protein (Protein is coded in complementary strand; from 1568247 to 1567069)
ORF.. 1568240-1569487	Sequence	P186_1864 - putative inner membrane protein (Protein is coded in complementary strand; from 1569487 to 1568240)