Sequence retrieval

Genome: Pyrobaculum sp. 1860
Start position: 1534269
End position: 1534745
Length: 477

DNA sequence
>NC_016645, from 1534269 to 1534745 (477 bp); Pyrobaculum sp. 1860 ATGAGGCTCGGCATGTGCAGAACCTCTTCGATTCTCGACTACCGGGCTGTGGTGTTTCTAGAGGGGGAGG GCTACGTCAGGGTCACCGCCGCAAACGGCAAGTCGCTTGTGGCGAAGGTGGTGAAGGGGCCTTGTTCGGG GGTTTCTAGGGAGGTGGCCTACCTCCTATACCCCAACTACGGCTGGGGCAGGGTGCCCGTGGAGGCGGAG TTCGCCGTGGAGGCTGTGGAGCCTGTGAAGGCTACTAGAGTTGTGATGCGGGTGCCCTTCGGCATAGGGG AGATCGTTGTGCGGAGACAGCTGGAGGGCTTCCCCGTGTACGAGGGGTCGGTGGCGCTTGAATATCTGGA GCATATAGAGTTCGGCGAAGTGGTGCACGTGGAGCCTGCCCAGTTCTCAGTGCTGGCGCCCGACACCAAG CTGAGGCTCGTGGAGGTTCCCGTGGACGACTCCGAGGTCGTCTTTATGCGTAGGTAG

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1533884-1534270	Sequence	P186_1821 - hypothetical protein (Protein is coded in complementary strand; from 1534270 to 1533884)
ORF.. 1534269-1534745	Sequence	P186_1822 - hypothetical protein