Sequence retrieval

Genome: Pyrobaculum sp. 1860
Start position: 1362306
End position: 1364132
Length: 1827

DNA sequence
>NC_016645, from 1362306 to 1364132 (1827 bp); Pyrobaculum sp. 1860 TCATAAAACCTCCACCACCTCCTCCAGCGCCTTGTAGACGTGGTGGCAGGGCCTCCCCGCGCCGCAGACG CTACACGTGGGCCTCCCGTCCTCCACCACCACCTCCACCTCCCTATCCCCCTCCAGTAGCTTCACCTTTA TGCGGCCTCCCTCCCTCAGCTTCTGCACCACCTTGAGCCTCGCCGCGGAGAGCATTACGCCGGGGCCCGG CGCCGAGTTGTACAGCCTCCGCCAGAGCTGCGCCACGTCGGCGGTTTTATCCACCCGCCACGCCTCCGCC AGGTCTATGTCCCCACCGCCGTACTCCAGCACTCTGTCTCTCACCTTCAGCACAACGGGGAGCCTCACCA GGGGCCCCAGCACTACAGCCTCCCCCACGTCGAGCCCCGGGAGGTTCTCCAGAAACTCCTGGGCGAGCAA CTCGCTGCTCTCCCTAACGGCCTCCTGGTCTCTTGGGTTGACGATGCGCATAATCACCTGGCTCTGGCAC TGGCTAATTATGTCGGGGTCCACCTTGCTCGGCCTCTGGGTGATGGCGACTAGGTAGGCCCCGAACTTCC GGCCTTCAGACGCCACCCGGGACAGGGCCTCGTAAGACCTAGTCTTCCGCAGAGACTTAGGCGGGGCGAA GCGGTGAGCCTCCTCTACGAAGAGCACCACGGGCCACGGCAACTTCACCCCCCTCAGCCCCCTCACGTGC CTCACCCTCGCCTGGAACAGCCTGTTGACGAGATGCGCCACGACGTAGTCCTGCACCTCCTCGTTGACCC CCGCCAGGTTGACGATGGTGATGTCCCCCAGCATCTTGGCGAGGGGAGTGGAGCGGGGGGCGAAGACCCC CAGCCTCTGGAGCCTCTTTAAATAAGTCAACGCGCTGAACACCGCGTGGCGGTCTTTAGAGGCTAACTGC TTGAGGTCGGACATGGGGAAGTCCCCCTCCAACCTGTACTGCTGGAACAGTTTGTTAAGCGCGCCCTCCC CCCTCATCGCCGTGTATAGGACCCGCCTAAGGAAGGCGAGGCCCACGGGCCTGCCGACGGCCTTCTTGAC CTTCTTGGCGTAGGACCAGGCGAGGTATATGGCGTATCTAATCTTCTTAGCGCCGGGGGGCACCCCCGCG GCGTTTGCCAAAGCCTCTGGATCGCTGTCTAGGACGCCGATTTTGTACATCAAGTCCCCTACGTGGTGCT CCGCCACCTTCACCACCCTCGCCGTGTAGGGGCCGAGGCGGTGGATGCTGTCTCTAATTGGGACGTACTC GCCGTGGGGGTCGATAACCACGATCTTGGCGCCTTTCTTCAACAACTCCTCTATGAGGACCACGGAGAAC CAAGTCTTCCCGCTACCAGTCGCCGCTATGACCGCTATGTGTCTCTTAACTCCGTTGAGGTCTAGACAGA TCTCCACGTCCCGGCTGGCGAGCCTGCCCACGCAGAGGCCGTTCTCGACTCTGAACAACTCCTCCACGTC GGCGTCCTCGGCGAGGTAGACGGAGGCGCCTATCCTGGGGGGTTGCTTCGGCTGCCTCAGCTCGCCCCCC TCTGCGTAGCCCAGCACCTTCACCACGGCTATCTGCACCTCCAGGATATTCTCAGACACGTCTCTAATCA CGCTGTACAGGGGGAGGTCCCTCTTGGCTGAGTAGGGGTCCCGGTACAGCTTGACTATCTGCCCCAGAAG CCGCGCCGAGACGTGCTCCCCCCTCCCCGGATCGTACTCCACGTGGTCAACCACTACGTAGTCGTACAGG CTGATGGGCTTCTCGGGGTCAAGCGTCGCCACGAACTCGAAGGGCGTCGCCGTCGCCACCACGTAGCCGA TTCTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1361152-1362309	Sequence	P186_1616 - metallophosphoesterase (Protein is coded in complementary strand; from 1362309 to 1361152)
ORF.. 1362306-1364132	Sequence	P186_1617 - putative ATPase (Protein is coded in complementary strand; from 1364132 to 1362306)
ORF... 1364129-1365418	Sequence	P186_1618 - hypothetical protein (Protein is coded in complementary strand; from 1365418 to 1364129)