Sequence retrieval

Genome: Pyrobaculum sp. 1860
Start position: 1051098
End position: 1051952
Length: 855

DNA sequence
>NC_016645, from 1051098 to 1051952 (855 bp); Pyrobaculum sp. 1860 GTGACATTCTCTATAGTGGCTACAGACGGCGTCGACGTAGGCGTCGCCGTGTCTTCAAAATTTGTCGCCG TAGGCGCGTTTGTACCCCACGTCGAGGCTTCTGTGGGTGCCGTGGCTACGCAGTGCTACGCTAATCCGCG GCTGGGCAAGCTAATACTGGCACTAATGAGGCAAGGCGTGCACGCTAGAGAGGCTGTGGAGAGGGCGGTG GCCCAGGACTCTGAGAGGGAGCTTAGACAGATAGGGGCTGTCGACGCCAGAGGCAACGCCTACGGCTTTA CCGGCCGGCAGTGCCCCGAGTACGCTGGTCATGTCGTTGGTAGCGGCTACGTGGCTCTAGGCAACATCTT GGCGGGGCCGCAGGTGGTTGAGGCCATGGCTAAGGCCTTTGAGACGCAGAGAGGGGAGCTGGTGGATAAG CTAATAGCCGCCTTAGAAGCCGGGGAGAGGGCGGGCGGAGACAGAAGGGGCAAGCAGTCGGCGGCGGTTG TTGTGCTAAGACCGGGAGGCGGCTACCTCGGCCTCACAGACGTGTACGTCGACATTAGGGTAGACGATCA CCCGGAGCCCGTGGCGGAGCTCAAAAGGATATTCAAGATATGGGAGCTGACTCTACTCCAGAGAGACGAC CCGAGAGACGTAGTGTTGAAAAAAGACGTGGCGCGGGAGGTGCAGGAAGCCTTGAGGCGGCTTGGGTTCT ACAAAAAAGAGCCGACGGGGGTGTGGGATGAGGAGACTGAGAAAGCCTTCAGAGACTGGGCTGGCTACGA AAACTTTGAGAACAAGATAAGAGGCGACGACAAGATCTGGGGCTCCCTCTACCGATACCTCATAGAGGCG TCAAGAAGGAGTTAA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1051098-1051952	Sequence	P186_1251 - major pilin protein fimA
ORF... 1051938-1052654	Sequence	P186_1252 - sugar fermentation stimulation protein (Protein is coded in complementary strand; from 1052654 to 1051938)