DNA sequence >NC_016593, from 2999255 to 2999317 (63 bp); Geobacillus thermoleovorans CCB_US3_UF5 ATCGAAGCGAGAGCGGGCAGCATCGAAGAGAGAGTGAACAGCATCGAGGCGAGAGTAGACAGC Translate to protein Restriction digest BLAST Design primers with primer3