DNA sequence >NC_016593, from 2187486 to 2187593 (108 bp); Geobacillus thermoleovorans CCB_US3_UF5 CAAGGACCCACAGGACCTCAAGGACCTACAGGGCCGACCGGACCCACAGGACCTCAAGGACCTACAGGGC CGACTGGACCCACAGGACCTCAAGGACCCACAGGGCCG Translate to protein Restriction digest BLAST Design primers with primer3