Sequence retrieval

Genome: Flavobacterium columnare ATCC 49512
Start position: 792750
End position: 793982
Length: 1233

DNA sequence
>NC_016510, from 792750 to 793982 (1233 bp); Flavobacterium columnare ATCC 49512 GTGGAAATAAAACTTCAAAAATCAGTTGTAAATAATGAACAACAGCCCATTAAAATAACAGGTAGCAAAT CAGAAACCAATAGGTTATTACTATTACAAGCGCTGTATCCAAATATTGTGGTAGAAAATATATCCAATTC GGACGATTCAGAGGTAATGCAAAAAGCTTTAAACGGTTCTTTTGAAGGGGTGGTAGACGTGCATCATGCT GGAACAGCTATGCGTTTTTTAACTGCCTTTTTTGCCATTCAAGAAGGAAAAGAAATAATACTAACGGGGT CTTCTCGTATGAAAGAGCGACCTATTAAAATTTTAGTAGAAGCTTTACAACAACTAGGTGTTGAAATTTT ATATTTAGAAAACGAAGGTTTTCCGCCTATTAAAATTAAAGGAAAAAAAATAACCCAAAATAAAGTAATA TTGCCCGCCCATGTAAGCAGTCAGTATATATCTGCTTTGCTCTTAATTGCTCCAACATTAGAGAAAGGAT TAGAGCTAACGTTAGAAGGACAGATTACTTCTATTCCGTATATCCAAATGACTTTATCATTGTTAAACGA AATGGGAGTTCAAACATCTTTTGAAGAAAATAACATTACAGTCAATCCGAAATCCGAAATCCAAAATCCA AAATTTACAGTTGAGTCTGATTGGAGTTCTGCATCTTATTGGTATTCTATTGTAGCACTTTCGGAATTAG GTACACAAATAACGCTTTGTAGTTACAAAAAAAATAGCTTACAAGGAGATGCCGTTCTGGCAGAAATTTA TCAGTATTTTGGTGTCGAAACTATTTTTAATAACAATACAATAATATTAAAAAAAAGAACACCTCATTCA GAATTTATAACGTATAACCTGAATACTTGCCCAGATATAGCCCAAACAATTGCGGTAACCTGTTTGGGCC TTGGAATAGGATGTGATTTGTATGGTTTGCATACCCTAAAGATTAAAGAAACAGACCGTTTAGAAGCCTT GAAATTAGAGTTCACTAAATTAGGTGCTGAAGTAATGGTTACTGCCGAATCATTACATTTAAAACCTTCC ACGTTTATTAATCAAAATATCAGCATAAAAACCTATCAAGATCACAGAATGGCAATGGCTTTTGCCCCGC TTGCACTTCAAGTACCTATTACAATAGAAAATGCTGAGGTAGTAAGTAAATCATATCCTTATTTTTGGAA AGATCTACAAACGGTAGGTTTTGTTTATACGAAAGTACTATGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 792750-793982	Sequence	FCOL_03415 - 3-phosphoshikimate 1-carboxyvinyltransferase