Sequence retrieval

Genome: Flavobacterium columnare ATCC 49512
Start position: 77750
End position: 78124
Length: 375

DNA sequence
>NC_016510, from 77749 to 78123 (375 bp); Flavobacterium columnare ATCC 49512 TTATTCTTCATCTTCACTGTCATCTTCACTTTCGTCCTCGTCATCAGAATCCTGATCATCAGAATCTTCT TCGTCTTTTTCTTCGTCTGATGCAGCGTCAGAGTCGATTTCTAAATCTTTTAATGCGTCTAGTGTGTCAT CACCATCGATATCGTCATCGTCGTCAAAATTCACAATTCGATCGGCTAATTTAGTGCTTACTTTTACTAA ATAAATAGTGTCTTCAGTTCTAACTTCAACAGCTTCGATTGTTTCATTTTTAGCATTTTTAAAACGAATG ATGTCAGAATCATCATAACCTTCAGGGAATTTCTCTACTAAAAGGTTTAAAATTTCACTCGTAAGCTTTG AATAATCTACAATAACTCTTTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 77750-78124	Sequence	FCOL_00345 - hypothetical protein (Protein is coded in complementary strand; from 78124 to 77750)