Sequence retrieval

Genome: Flavobacterium columnare ATCC 49512
Start position: 645957
End position: 646256
Length: 300

DNA sequence
>NC_016510, from 645957 to 646256 (300 bp); Flavobacterium columnare ATCC 49512 TTATTTATTATTATTTCTTTTTTTTTCTTCTTCTATGAGTTCTTGAATTTTTCGCTCTTCCCAATTCTTA CTCATAAAAGAAAAGTCATAAACTTTTAAACCATGAAAAAACAAGCCTATCCCCCAACCTAGCATTGGAT AAAAAAACCAAATATGATTTGATGAATATTTTAGATTTATACAAATGAAAAAAGTATTTATACATAAATA AGAAATAAGATGTGAGTAAAAATCTTTGATTTCTTTTACTTTTTTCCTTGCTTTTTCATAACGTTGAAAA TCATCATTAAAATTTTCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 645957-646256	Sequence	FCOL_02820 - hypothetical protein (Protein is coded in complementary strand; from 646256 to 645957)