Sequence retrieval

Genome: Flavobacterium columnare ATCC 49512
Start position: 561197
End position: 562450
Length: 1254

DNA sequence
>NC_016510, from 561197 to 562450 (1254 bp); Flavobacterium columnare ATCC 49512 TTATTCATTTCTTTTTTTTTTTGCTGTTAATATGGTAATTGGATTATGTGTATCTATCGTTATTTCTATT GGTTCTAATAAATCGTAATTTAAGTTTTTAACACTCTTTATAAAGCATTCTTTACTTTCTTTTTTTACGG CATTTATTACAATTCTTCCATTTAATAAAAGGTATTGATCTATTAATAGAATAAGTTCTTCTAATTTGTT TCCATGACCACCAATAAAAACGCTATCTGGAACAGGAAGTGTACTTAAATCTATTTCAAAAAAATCACCT ATTATTTTAGTGATTCCTGGTGTGGAATGTTTTTTTGTATTGATATCAAAGATGGTACTGCATTCTTCTC GTTTTTCAAAGGCAATACTGTTTAAATGTGGAAATTGTGTTTTTGTTTCAATAGATACGGATCCTGTACA AAATCCTATATCCCAAAATGTTTTTTTATTGTGTAAATCTAATTGAGAAAGACTAATTAATCGTATAGGC ATTTTGGTAATCATGTTGGGACGATTTTCTAAACCTATAAATTCAATATCTCTAATTCCAAATTTTTTTT GTTTTTCTTCTTTTTTTATAAGTAACAAACAATTTAGTACATCAAATGTTTTTTTTGTTGCTTCTATTAG ACTTAAAGTGGTTATTTTTTCATTTTCTGCTTCCAATGCTTCTCCTATAATCATTTCATAGTTATTAAAA TTGTAGTCGAGCATTCGTTGAGCTATTATACTTGGCGTTTTAATATGATCTGTTAAAACGCCTATTAGGC TTGATTGATTAATAAGTGCTATATCTAGTTCTTTCCATGAACGTCCATGAATTGAGGTATTAACTATATT TTGATATGGAATGGTTTTTTTTTGACATAATAGTTGTAAACTATTAAAGCTGGGGTATACTTTTATGGTT GCATTTGGATGATTTTTTTTTATAGTATTGGCAAAGCCATAAAATAAAGGATCTCCAGAGGCAAAAACAA TTATGGGTTTATTTATTTTTTTATATTGATCAAAAAGTAGAGGCATTTCTTTCGTAATTTCAATCCATTG ATGATTAATTGGAAGTTTTTCTTTTATTAATTCATAGTGTCTTTTGCCTCCTGAAAAAACGTGATGTTCT TTTACTATTTTTTTTACTTCATCGCTTAGAATAAAATGAGGATTATTAGGAATTCCAATAACGTAATAAT CTATAGTCGGCAATTGACAATTATGAGTATTAAATGGTAATGTAAAGGATAAATTATGAGTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 559369-561204	Sequence	FCOL_02415 - precorrin-4 C(11)-methyltransferase (Protein is coded in complementary strand; from 561204 to 559369)
ORF.. 561197-562450	Sequence	FCOL_02420 - precorrin-6y C5,15-methyltransferase subunit CbiE (Protein is coded in complementary strand; from 562450 to 561197)