Sequence retrieval

Genome: Flavobacterium columnare ATCC 49512
Start position: 37991
End position: 38737
Length: 747

DNA sequence
>NC_016510, from 37990 to 38736 (747 bp); Flavobacterium columnare ATCC 49512 TTGAGAGGCATTTTTTTTATTTTTATCTTTTCTTTTGTTATTGATTTTAAGAATTTTAGTATTTATGAAG AACTTTTATCTATTTGTTTTTTTATTTCGTTTTTTGGTTTTTCACAAAATTTGCCTGAAATAAAAGTATT GGATTCATTGTATAGAGAAGATCAATTCTATCTAGGGATTACTTATAATATACTTAATAATAAACCACAA GGGGTTTCTCAAAATTCAACTTCAGGAAGTATTTTTTTGGGTTTTTTACGAGATATGCCTATTAATAAAT TGCGTACCTTGGCGATTGCTCCAGGTATTGGTTTTTCTTATTCTAATTTTAAACAAAATTTACTTATTAA AGATAATGGGGAAAGTATTGATTATTCAATTATTTCTAAAGATGAAATGTATGATAAAAATCGTTTTTCA TTTGTAACACTAGATGCTCCATTAGAGTTTCGTTGGCGTAATTCTACTCCTCAAAGTCATCGTTTTTGGA GAATTTATGGGGGAATAAAGGCAAGTTATGTCTTATACAATCAATCTCAGTTTATTAATTCTAAATATGA ATATAAAGTAACAAATAATCCAGATTTTAATCGTTTTCTTTATTCTGTTTTTCTGACAGCGGGATATAAT AGTGGGAATTTTTATGTATCTTATGGATTAAACGATTTTTTTAGAAAATCCTTTTTTAACGAAAATTCAG GGAAATTTCGCTCTTTGAATTTAGGATTGATGTTTTATATTTTATAA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 37991-38737	Sequence	FCOL_00195 - hypothetical protein